Lactolisterin BU, a novel Class II broad spectrum bacteriocin from Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4.

Lactolisterin BU, a novel Class II broad spectrum bacteriocin from Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4. Appl Environ Microbiol. 2017 Aug 25;: Authors: Lozo J, Mirkovic N, O'Connor PM, Malesevic M, Miljkovic M, Polovic N, Jovcic B, Cotter PD, Kojic M Abstract Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 produces a novel bacteriocin, lactolisterin BU, with strong antimicrobial activity against many species of Gram-positive bacteria, including important food spoilage and food-borne pathogens such as Listeria monocytogenes, Staphylococcus aureus, Bacillus sp. and streptococci. Lactolisterin BU was extracted from the cell surface of BGBU1-4 by propan-2-ol and purified to homogeneity by C18 solid phase extraction and reversed phase HPLC. The molecular mass of the purified lactolisterin BU was 5160.94 Da and an internal fragment, AVSWAWQH, as determined by N-terminal sequencing, showed low level similarity with existing antimicrobial peptides. Curing and transformation experiments revealed the presence of a corresponding bacteriocin operon on the smallest plasmid pBU6 (6.2 kb) of strain BGBU1-4. Analysis of the bacteriocin operon revealed a leaderless bacteriocin of 43 amino acids that exhibited similarity to bacteriocin BHT-B (63%), from Streptococcus ratti, a bacteriocin with analogy to aureocin A.IMPORTANCE Lactolisterin BU, broad spectrum leaderless bacteriocin produced by L. lactis subsp. lactis bv....
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research