Humanized mice to study human hematopoietic stem cell function in vivo

Xenotransplantation models enable the in-depth analysis of human hematopoietic stem cell (HSC) function in vivo.We generated novel mouse models supporting stable HSC engraftment, which is a prerequisite for the continuous generation of all adult human hematopoietic cell types in mice. By introducing a loss-of-function Kit receptor into NOD/SCID Il2rg-/- (NSG) mice we generated NOD/SCID Il2rg-/- KitW41/W41 (NSGW41) mice that combine an impaired endogenous HSC compartment with immunodeficiency that efficiently support stable engraftment of human HSCs in the long-term without the need for any conditioning therapy.
Source: Experimental Hematology - Category: Hematology Authors: Source Type: research