Activation of Vimentin is Critical to Promote a Metastatic Potential of Cholangiocarcinoma Cells.

Activation of Vimentin is Critical to Promote a Metastatic Potential of Cholangiocarcinoma Cells. Oncol Res. 2017 Jul 25;: Authors: Saentaweesuk W, Araki N, Vaeteewoottacharn K, Silsirivanit A, Seubwai W, Talabnin C, Muisuk K, Sripa B, Wongkham S, Okada S, Wongkham C Abstract Cholangiocarcinoma (CCA) is a highly metastatic tumor, of which the majority of the patients have short survival due tono effective treatment. Hence, a better understanding regarding CCA metastasis may provide an opportunity to improve the strategies for treatment. A comparison study between the highly metastatic cells and their parental cells is an approach to uncover the molecular mechanisms underlying the metastatic process. In the present study, a lung metastatic CCA cell line, KKU-214L5 was established by the in vivo selection of the tail-vein injected mouse model. KKU-214L5 cells possessed mesenchymal spindle-like morphology with higher migration and invasion abilities in vitro than the parental cells, KKU-214. KKU-214L5 also exhibited extremely aggressive lung colonization in the tail-vein injected metastatic model. Epithelial-mesenchymal transition (EMT) was clearly observed in KKU-214L5 cells. Significant down-regulation of epithelial markers, ZO-1 and claudin-1, with unique upregulation of E-cadherin and mesenchymal markers, vimentin, β-catenin, and slug were observed in KKU-214L5. Increasing of MMP-2 and -9 activities and CD147 expression ref...
Source: Oncology Research - Category: Cancer & Oncology Tags: Oncol Res Source Type: research