Biological control of Septoria leaf blotch and growth promotion in wheat by Paenibacillus sp. strain B2 and Curtobacterium plantarum strain EDS

Publication date: Available online 21 July 2017 Source:Biological Control Author(s): Erika Samain, Diederik van Tuinen, Philippe Jeandet, Thierry Aussenac, Sameh Selim Many of the non-pathogenic endophytic bacteria that reside in plant roots promote plant growth as well as protection against pathogens attack. However, little is known about their mode of action in wheat. We have previously demonstrated the potential of Paenibacillus sp. strain B2 (PB2) to stimulate plant defense mechanisms via its paenimyxin lipo-polypeptide elicitor. Recently, we isolated the Curtobacterium plantarum strain EDS (EDS) from seeds of almost all wheat cultivars. In the present work, the ability of PB2 and EDS to promote wheat growth and protection against Septoria leaf blotch (SLB) was investigated. Results showed that PB2 is a general root external colonizer and cultivar-dependent endophyte. In the endophytic state and only in co-inoculation, it significantly increased the internal root colonization by EDS, resulting in an increase of roots and aerial part fresh weights. qPCR analysis showed that, in the endophytic and nonendophytic states, PB2 conferred ≥59% protection against SLB by inducing systemic resistance which is characterized by the over expression of the pr1, lox, Aos, peroxidase, oxo and gst genes. Paenimyxin conferred 76% local protection characterized by the overexpression of the glu, lox, aos, pal, chs, oxo, and gst genes, and 82% systemic protection by chs. It was conclu...

Source: Biological Control - July 22, 2017 Category: Biology Source Type: research