Circulating Sex Steroids Co-regulate Adipose Tissue Immune Cell Populations in Healthy Men.

Circulating Sex Steroids Co-regulate Adipose Tissue Immune Cell Populations in Healthy Men. Am J Physiol Endocrinol Metab. 2017 Jul 11;:ajpendo.00075.2017 Authors: Rubinow KB, Chao J, Hagman D, Kratz M, Van Yserloo B, Gaikwad NW, Amory JK, Page ST Abstract Male hypogonadism results in changes in body composition characterized by increases in fat mass. Resident immune cells influence energy metabolism in adipose tissue and could promote increased adiposity through paracrine effects. We hypothesized that manipulation of circulating sex steroid levels in healthy men would alter adipose tissue immune cell populations. Subjects (n=44 men, 19-55 years of age) received 4 weeks of treatment with the GnRH receptor antagonist acyline with daily administration of 1) placebo gel, 2) 1.25g testosterone gel (1.62%), 3) 5g testosterone gel, or 4) 5g testosterone gel with an aromatase inhibitor. Subcutaneous adipose tissue biopsies were performed at baseline and end-of-treatment, and adipose tissue immune cells, gene expression, and intra-adipose estrogen levels were quantified. Change in serum total testosterone level correlated inversely with change in the number of CD3+ (β=-0.36, p=0.04), CD4+ (β=-0.34, p=0.04), and CD8+ (β=-0.33, p=0.05) T cells within adipose tissue. Change in serum 17β-estradiol level correlated inversely with change in the number of adipose tissue macrophages (ATMs) (β=-0.34, p=0.05). A negative association also was foun...
Source: American Journal of Physiology. Endocrinology and Metabolism - Category: Physiology Authors: Tags: Am J Physiol Endocrinol Metab Source Type: research