Evaluation of histopathological changes, viral load and immune function of domestic geese infected with Newcastle disease virus.

Evaluation of histopathological changes, viral load and immune function of domestic geese infected with Newcastle disease virus. Avian Pathol. 2014 Jun 9;:1-28 Authors: Lu A, Diao Y, Chen H, Wang J, Ge P, Sun X, Hao D Abstract Outbreaks of Newcastle disease (ND) in flocks of geese with high morbidity and mortality in Southern and Eastern China have been reported frequently since late 1990s which broke the traditional view that geese are considered to be the natural reservoir of ND virus (NDV) and show few or no clinical signs after infection. In this present study, geese were infected intranasally. Clinical disease and gross pathology were observed. Serum and immune organs were collected from geese sequentially euthanized or after disease-associated death. We studied the histopathology of immune organs by haematoxyln hosin (HE) staining and NDV fusion protein was detected in tissues by immunohistochemistry (IHC). At the same time, the SYBR Green I real-time polymerase chain reaction (PCR) assay was used to detect the viral load from the collected samples. Serum samples were tested for NDV-specific antibodies and avian influenza virus (AIV)-specific antibodies by haemagglutination inhibition (HI) test. The results showed that serious lesions and numerous positive reaction of NDV antigen were detected in the immune organs. High viral load in immune organs was developed in infected geese, correlating with the severity of clinical signs ...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research