In vitro uptake and toxicity studies of metal nanoparticles and metal oxide nanoparticles in human HT29 cells.

In vitro uptake and toxicity studies of metal nanoparticles and metal oxide nanoparticles in human HT29 cells. Arch Toxicol. 2017 May 02;: Authors: Schneider T, Westermann M, Glei M Abstract In this paper, we investigated the toxicological behavior of metal nanoparticles (gold, silver) and metal oxide nanoparticles (copper oxide, zinc oxide, titanium dioxide) in vitro in human colorectal adenocarcinoma cells (HT29). We analyzed the cellular uptake by ICP-MS and TEM, the influence on cell viability by MTT assay and trypan blue exclusion test, their effect on DNA damage and/or generation of oxidized bases by alkaline comet assay, and their potential to induce apoptosis by flow cytometry after 24-h nanoparticle treatment with concentrations between 2 and 10 µg/ml. We determined the amount of metal taken up by a single HT29 cell, ranging from 0.02 pg/cell up to 1.39 pg/cell. Cell viability assays showed a significantly decrease for metal oxide nanoparticles using trypan blue exclusion test and for all nanoparticles, except titanium dioxide, using MTT assay. Genotoxic effects after nanoparticle treatment were not observed for the tested concentrations. Apoptosis induction was significantly increased for silver nanoparticles (tested for two sizes) as well as for titanium dioxide and zinc oxide nanoparticles. Our results indicate potential health risks of oral NP uptake by food ingredients or food contamination, making further mechanist...
Source: Archives of Toxicology - Category: Toxicology Authors: Tags: Arch Toxicol Source Type: research