PAR1 ‑mediated c‑Jun activation promotes heat stress‑induced early stage apoptosis of human umbilical vein endothelial cells.

PAR1‑mediated c‑Jun activation promotes heat stress‑induced early stage apoptosis of human umbilical vein endothelial cells. Mol Med Rep. 2017 Mar 09;: Authors: Zhang S, Liu Y, Wang Z, Liu J, Gu Z, Xu Q, Su L Abstract Our previous study indicated that when human umbilical vein endothelial cells (HUVECs), which are involved in endothelial barrier function, are heat stressed, levels of protease‑activated receptor 1 (PAR1) are increased significantly. In the present study, it was demonstrated that PAR1 serves a vital role in heat stress‑induced HUVEC apoptosis. When the PAR1 inhibitor, SCH79797 (SCH), or a small interfering RNA (siRNA) targeting PAR1 were used to inhibit PAR1 signaling, a marked decrease in cell apoptosis, caspase‑3 activity and the expression of the pro‑apoptotic protein B‑cell lymphoma 2 (Bcl‑2) associated X (Bax), as well as increased expression of the anti‑apoptotic Bcl‑2 family member, myeloid cell leukemia 1 (Mcl‑1), were observed. In addition, heat stress‑induced apoptosis, caspase‑3 activity and the expression of Bax were significantly increased following administration of the PAR1 agonist, TFLLR‑NH2 or adenovirus overexpression of PAR1. This was accompanied by decreased protein expression levels of Mcl‑1. Furthermore, it was identified that the DNA binding activity of the nuclear factor (NF)‑κB p65 subunit increased and c‑Jun activation was reduced as a result of inhibition ...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research