Digital gene expression profiling of the pathogen-resistance mechanism of Oryza sativa 9311 in response to Bacillus amyloliquefaciens FZB42 induction

Publication date: Available online 18 April 2017 Source:Biological Control Author(s): Yongli Xie, Liming Wu, Bichun Zhu, Huijun Wu, Qin Gu, Faheem Rajer, Xuewen Gao Plant growth-promoting rhizobacteria (PGPR) are beneficial soil microorganisms that colonize plant roots for nutritional purposes and benefit plants by increasing plant growth or reducing plant disease. To clarify the complex mechanisms underlying plant-PGPR interactions, transcriptomic analyses of the response of rice (Oryza sativa) 9311 to Bacillus amyloliquefaciens FZB42 were carried out using RNA-Seq technology. This resulted in the identification of 379 differentially expressed genes in rice roots and 719 in rice leaves following FZB42 interaction that are likely to be related to plant-PGPR interactions, plant stress-resistance, pathogen-resistance, growth and development and basic and energy metabolism. We focused on plant disease-resistance mechanisms induced by FZB42. Variations in the expression of crucial genes COI1, MEKK1, WRKY, and PR that are involved in signal transduction following plant-pathogen interaction at different interaction time points were examined. Significantly up-regulated expression of PR, LOX, and ERF genes involved in the salicylic acid, jasmonic acid, and ethylene signal transduction pathways, respectively, were detected, suggest that defense reactions in rice were induced following interaction with FZB42. Phytoalexin changes in rice induced by FZB42 were examined and momil...

Source: Biological Control - April 19, 2017 Category: Biology Source Type: research