Enhancement of 2,3-butanediol production from Jerusalem artichoke tuber extract by a recombinant Bacillus sp. strain BRC1 with increased inulinase activity.

Enhancement of 2,3-butanediol production from Jerusalem artichoke tuber extract by a recombinant Bacillus sp. strain BRC1 with increased inulinase activity. J Ind Microbiol Biotechnol. 2017 Mar 18;: Authors: Park JM, Oh BR, Kang IY, Heo SY, Seo JW, Park SM, Hong WK, Kim CH Abstract A Bacillus sp. strain named BRC1 is capable of producing 2,3-butanediol (2,3-BD) using hydrolysates of the Jerusalem artichoke tuber (JAT), a rich source of the fructose polymer inulin. To enhance 2,3-BD production, we undertook an extensive analysis of the Bacillus sp. BRC1 genome, identifying a putative gene (sacC) encoding a fructan hydrolysis enzyme and characterizing the activity of the resulting recombinant protein expressed in and purified from Escherichia coli. Introduction of the sacC gene into Bacillus sp. BRC1 using an expression vector increased enzymatic activity more than twofold. Consistent with this increased enzyme expression, 2,3-BD production from JAT was also increased from 3.98 to 8.10 g L(-1). Fed-batch fermentation of the recombinant strain produced a maximal level of 2,3-BD production of 28.6 g L(-1), showing a high theoretical yield of 92.3%. PMID: 28315963 [PubMed - as supplied by publisher]
Source: Journal of Industrial Microbiology and Biotechnology - Category: Microbiology Authors: Tags: J Ind Microbiol Biotechnol Source Type: research