Starch-derived absorbable polysaccharide hemostat enhances bone healing via BMP-2 protein.

In this study, we used a model of rabbit parietal bone defect and a mouse osteoblast cell line MC3T3-E1 to evaluate the effects of SAPH on bone healing. We found that SAPH significantly decreased bone healing scores, reduced defective area of parietal bone, and increased the areas of bone trabeculae and cavitas medullaris. In addition, SAPH enhanced MC3T3-E1 osteoblasts proliferation, up-regulated the expressions of alkaline phosphatase (ALP) and osteocalcin and increased the level of bone morphogenetic protein 2 (BMP-2) in MC3T3-E1 osteoblasts. These SAPH-induced benefits in MC3T3-E1 osteoblasts were significantly abolished by the application of BMP-2-siRNA. These findings suggested that SAPH enhances bone healing, promotes the proliferation, differentiation and maturation of osteoblast by up-regulating BMP-2 expression in osteoblastic cells.
PMID: 28168995 [PubMed - as supplied by publisher]

Source: Acta Histochemica - February 2, 2017 Category: Biochemistry Authors: Niu W, Wang Y, Liu Y, Zhang B, Liu M, Luo Y, Zhao P, Zhang Y, Wu H, Ma L, Li Z Tags: Acta Histochem Source Type: research