Rv1458c: A new diagnostic marker for identification of M. tuberculosis complex in a novel duplex PCR assay.

Rv1458c: A new diagnostic marker for identification of M. tuberculosis complex in a novel duplex PCR assay. J Med Microbiol. 2017 Jan 23;: Authors: Shrivastava K, Garima K, Narang A, Bhattacharyya K, Vishnoi E, Singh RK, Choudhary A, Prasad R, Bose M, Varma-Basil M Abstract We explored the efficiency of Rv1458c, a putative ABC drug transporter specific for the Mycobacterium tuberculosis complex (MTBC) as a diagnostic marker. A 190 bp region of Rv1458c and a 300bp region of hsp65 were targeted in a novel duplex PCR assay and compared with PCR restriction analysis (PRA) using restriction enzymes NruI and BamHI. Speciation of a subset of the isolates (n=50) was confirmed by sequencing. Clinical isolates (n=426) obtained from clinically suspected patients of pulmonary tuberculosis; and mycobacterial (n=13) and non-mycobacterial (n=8) reference strains were included in the study. The duplex PCR correctly identified 320/426 isolates as MTBC and 106/426 isolates as nontuberculous mycobacteria (NTM). The test was 100% specific and sensitive when compared with NruI/BamHI PRA and highlighted the use of Rv1458c as a diagnostic marker for MTBC. The assay could be developed to be used as a screening test to identify MTBC in clinical specimens in peripheral laboratories with frugal resources. PMID: 28126044 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research