Evolutionary and genetic analysis of human bocavirus genotype-1 strains reveals an evidence of intragenomic recombination.

Evolutionary and genetic analysis of human bocavirus genotype-1 strains reveals an evidence of intragenomic recombination. J Med Microbiol. 2017 Jan 11;: Authors: Abdel-Moneim AS, Kamel MM, Hassan NM Abstract Human bocavirus (HBoV) is divided into 4 genotypes: 1 to 4 with the HBoV-1 is the most commonly prevalent genotype. The current study intended to genetically analyze the full length genome of the HBoV-1 of recently detected Egyptian strains. Seven overlapping sets of primers were developed to amplify a nearly complete HBoV-1 genome from the clinical samples. The primer sets were tested on three recently identified Egyptian HBoV-1 strains with viral loads ≥105/ml. Sequencing was conducted using the same sets of primers. HBoV-1 virus strains were genetically analysed based on the sequences of their complete genomes and the individual open reading frames. The new sets of primers successfully amplified the three tested strains. Sequence analysis of the full-length genome of the HBoV-1 revealed a considerable level of genetic heterogenicity between different strains. Based on the full-genome and VP1 open reading frame, HBoV-1 viruses were clustered into three main lineages A to C and the lineage A is further subdivided into three sublineages A1-A3. The Egyptian strains were clustered within two sublineages: A1 and A2. New amino acid substitutions were detected in NS1 and VP1/VP2 proteins. Both inter and intragenomic recombination e...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research