Fast and sensitive analysis of beta blockers by ultra-highperformance liquid chromatography coupled with ultrahigh-resolution TOF mass spectrometry.

Fast and sensitive analysis of beta blockers by ultra-highperformance liquid chromatography coupled with ultrahigh-resolution TOF mass spectrometry. Biomed Chromatogr. 2016 Dec 07;: Authors: Tomková J, Ondra P, Kocianová E, Václavík J Abstract This paper presents a method for the determination of acebutolol, betaxolol, bisoprolol, metoprolol, nebivolol and sotalol in human serum by liquid-liquid extraction (LLE) and ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry (UHPLC-UHR-TOF MS). After liquid-liquid extraction, beta blockers were separated on a reverse phase analytical column Acclaim RS 120 (100 × 2.1 mm, 2.2 µm). The total run time was 6 min for each sample. Linearity, limit of detection (LOD), limit of quantification (LOQ), matrix effects, specificity, precision, accuracy, recovery and sample stability were evaluated. The method was successfully applied to the therapeutic drug monitoring (TDM) of 108 patients with hypertension. This method was also used for determination of beta blockers in 33 intoxicated patients. PMID: 27925253 [PubMed - as supplied by publisher]
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research