Nerve growth factor-carbon nanotube complex exerts prolonged protective effects in an in vitro model of ischemic stroke

Publication date: Available online 2 December 2016 Source:Life Sciences Author(s): Parichehr Hassanzadeh, Elham Arbabi, Fatemeh Atyabi, Rassoul Dinarvand Aims The therapeutic potential of nerve growth factor (NGF) against the neurological disorders may be negatively affected by its short half-life. Based on the superior properties of carbon nanotubes (CNTs) for controlled drug delivery, we aimed to prepare CNT-NGF complex and evaluate its effect in an in vitro model of ischemic stroke. Materials and methods Multi-walled CNTs (MWCNTs)-NGF complex was prepared using amino-functionalized COOH-MWCNTs and characterized by Fourier transform infrared spectroscopy and transmission electron microscopy. PC12 cells in the absence or presence of NGF (0.5, 1, 2μg/ml), acid- or amine-modified MWCNTs, or MWCNTs-NGF complex (2, 4, 8μg/ml) were exposed to 1 and 6h oxygen-glucose deprivation (OGD) followed by 24h re-oxygenation. Cytotoxicity and oxidative stress were evaluated. Key findings OGD significantly reduced the cell viability (P <0.001). NGF dose-dependently increased the cell viability only after 1-h OGD (P <0.05), while, MWCNTs-NGF complex was effective at both 1- and 6-h OGD (P <0.05, P <0.001). NGF reduced the formation of condensed nuclei due to 1-h OGD (P <0.01, P <0.001), while, MWCNTs-NGF showed efficiency at both time points (P <0.05, P <0.01, P <0.001). OGD significantly increased malondialdehyde (MDA) ...
Source: Life Sciences - Category: Biology Source Type: research