Oxaloacetate and Adipose stromal cells-conditional medium synergistically protected potassium/serum deprivation-induced neuronal apoptosis.

In this study, we found that ASC-CM contained glutamate oxaloacetate transaminase and its substrate, oxaloacetate (OAA) directly protected cerebellar granule neurons (CGN) from apoptosis induced by serum and potassium deprivation. Additionally, OAA inhibited serum and potassium deprivation-induced caspase 3 activation. ASC-CM and OAA in combination had a synergistic neuroprotective effect. Clearly, different from ASC-CM-induced neuroprotection, OAA-induced neuroprotection was Akt- independent but JNK-dependent. These data establish a mechanistic basis supporting that the application of ASC-CM for neuroprotective treatments could be significantly enhanced by addition of OAA.
PMID: 27816553 [PubMed - as supplied by publisher]

Source: Brain Research Bulletin - November 1, 2016 Category: Neurology Authors: Liu Q, Zhao G, Zhou C, Farlow MR, Du Y, Xu G, Gu H Tags: Brain Res Bull Source Type: research