Expression, purification, crystallization and preliminary crystallographic study of the cytoplasmic domain of the mitochondrial dynamics protein MiD51

In this study, the cytoplasmic domain of MiD51 was overexpressed in Escherichia coli, purified and crystallized. An X-ray diffraction data set was collected to a resolution of 3.1 Å and the crystal belonged to space group P41212, with unit-cell parameters a = b = 90.1, c = 124.7 Å, α = β = γ = 90°. The asymmetric unit had the highest probability of containing one molecule, with a Matthews coefficient of 3.32 Å3 Da−1 and a solvent content of 63.0%.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: mitochondrial dynamics mitochondrial fission mitochondrial dynamics protein MiD51 crystallization communications Source Type: research