In Vivo Cell Transfer Assay to Detect Autoreactive T Cell Subsets

Among the methods used in molecular biology, in vitro biochemical assays are more common, whereas in vivo assays, including the use of animal models, are less widely employed. In our studies on systemic lupus erythematosus (SLE), we have identified a novel T cell subtype termed “autoantibody-inducing CD4 T cells” (aiCD4 T cell) that is responsible for the development of autoimmunity. In order to identify and isolate these cells, we developed a new technique that involves the transfer of candidate T cell subpopulations into naïve mice and assaying for the development of autoantibodies in the recipient mice.
Source: Springer protocols feed by Molecular Medicine - Category: Molecular Biology Source Type: news