A comparative evaluation of the serological and molecular methods for the diagnosis of scrub typhus in Indian settings.

A comparative evaluation of the serological and molecular methods for the diagnosis of scrub typhus in Indian settings. Jpn J Infect Dis. 2016 Aug 31; Authors: Gupta N, Chaudhry R, K Kabra S, Lodha R, R Mirdha B, K Das B, Dey AB, Sreenivas V Abstract The major brunt of Scrub typhus is borne by the developing countries like India but the disease still remains neglected. The rapid progression to fatal complications and associated mortality calls for improved diagnostic services. IgM Immunofluorescence assay (IFA) with all its limitations is still the gold standard. Out of 218 suspected cases enrolled in the 2 year study period, 30 cases of scrub typhus were detected by IgM IFA at 1:64 dilution. The sensitivity of IgM Enzyme linked immunosorbant assay (ELISA), IgM Rapid Flow assay (RFA) and IgG RFA was found to be 97%, 87% and 77% respectively. Their respective specificity were 100%, 100% and 92% respectively. The sensitivity and specificity of Nested Polymerase Chain Reaction assay targeting 56kDa gene was found to be 50% and 100% respectively. PMID: 27580576 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research