Herpes Simplex Virus Mutant Generation and Dual-Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo

Two important components to a useful strategy to examine viral gene regulation in vivo are (1) a highly efficient protocol to generate viral mutants that limits undesired mutation and retains full replication competency in vivo and (2) an efficient system to detect and quantify viral promoter activity in rare cells in vivo. Our strategy and protocols for generating, characterizing, and employing HSV viral promoter/reporter mutants in vivo are provided in this two-part chapter.
Source: Springer protocols feed by Infectious Diseases - Category: Infectious Diseases Source Type: news