Rapid and accurate detection of carbapenemase genes in Enterobacteriaceae with the Cepheid Xpert Carba-R Assay.

Rapid and accurate detection of carbapenemase genes in Enterobacteriaceae with the Cepheid Xpert Carba-R Assay. J Med Microbiol. 2016 Jul 19; Authors: Smith M, Diederen B, Scahrringa J, Leversteijn-van Hall M, Fluit AC, Cohen Stuart J Abstract Rapid and accurate detection of carbapenemase-producing Enterobacteriaceae is pivotal for adequate antibiotic therapy and infection control. The Cepheid Xpert Carba-R Assay detects and identifies the most prevalent carbapenemases (KPC, VIM, IMP, NDM and OXA-48), using automated real-time PCR. The test performance of the Xpert Carba-R was evaluated with 129 well-characterized non-repeat Enterobacteriaceae isolates, suspected for carbapenemase production, i.e. with meropenem MICs > 0,25 mg/L. The isolate collection contained 100 carbapenemase producing isolates (36 KPC-2 or KPC-3, 20 VIM-1, 4 KPC-2 plus VIM-1, 5 NDM, 3 IMP, 1 IMP plus VIM-1, 31 OXA-48-like carbapenemases) and 29 negative control isolates producing ESBL and/or AmpC beta-lactamases. PCR and sequencing of beta-lactamase genes was used as reference test. The sensitivity of the Xpert Carba-R was 100% (100/100), with a 100% (29/29) specificity. The time to result was approximately 55 minutes with a hands-on time of only 1 minute per isolate. In conculsion, the Carba-R Assay is a rapid and accurate instrument for the confirmation and identification of the blaKPC, blaVIM, blaIMP, blaNDM and blaOXA-48 genes. PMID: 27451855 [Pub...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research