Assessment of the antibacterial, cytotoxic, and mutagenic potential of the phenolic-rich hydroalcoholic extract from Copaifera trapezifolia Hayne leaves.

Assessment of the antibacterial, cytotoxic, and mutagenic potential of the phenolic-rich hydroalcoholic extract from Copaifera trapezifolia Hayne leaves. J Med Microbiol. 2016 Jul 18; Authors: Leandro LF, Moraes TS, de Oliveira PF, Alves JM, Senedese JM, Ozelin SD, Resende FA, De Grandis RA, Varanda EA, Bastos JK, Tavares DC, Martins CH Abstract Copaifera trapezifolia Hayne occurs in the Atlantic Rainforest, which is considered one of the most important and endangered tropical forests on the planet. Although literature works have described many Copaifera species, their biological activities remain little known. In the present study, we aimed to evaluate (1) the potential of the hydroalcoholic extract from C. trapezifolia leaves (CTE) to act against the causative agents of tooth decay and apical periodontitis and (2) the cytotoxicity and mutagenicity of CTE to ensure that it is safe for subsequent application. Concerning the tested bacteria, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of CTE varied between 100 and 400 µg mL-1. The time-kill assay conducted at a CTE concentration of 100 µg mL-1 evidenced bactericidal activity against Porphyromonas gingivalis (ATCC 33277) and Peptostreptococcus micros (clinical isolate) within 72 h. CTE at 200 µg mL-1 inhibited P. gingivalis and P. micros biofilm formation by at least 50%. A combination of CTE with chlorhexidine dichlorohydrate did not ...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research