Sorghum bicolor < /em > ) on some cholinergic (acetylcholinesterase (AChE)) and purinergic (Na < sup class= " a-plus-plus " > + < /sup > /K < sup class= " a-plus-plus " > + < /sup > -ATPase and ecto-5 ′-nucleotidase) enzymes associated with neurological condition..."> Sorghum bicolor < /em > ) on some cholinergic (acetylcholinesterase (AChE)) and purinergic (Na < sup class= " a-plus-plus " > + < /sup > /K < sup class= " a-plus-plus " > + < /sup > -ATPase and ecto-5 ′-nucleotidase) enzymes associated with neurological condition..." /> Sorghum bicolor < /em > ) on some cholinergic (acetylcholinesterase (AChE)) and purinergic (Na < sup class= " a-plus-plus " > + < /sup > /K < sup class= " a-plus-plus " > + < /sup > -ATPase and ecto-5 ′-nucleotidase) enzymes associated with neurological condition..." />

Sorghum stem extract modulates Na + /K + -ATPase, ecto-5 ′-nucleotidase, and acetylcholinesterase activities

This study sought to assess the antioxidant activity and effects of phenolic extract from sorghum stem ( < em class= " a-plus-plus " > Sorghum bicolor < /em > ) on some cholinergic (acetylcholinesterase (AChE)) and purinergic (Na < sup class= " a-plus-plus " > + < /sup > /K < sup class= " a-plus-plus " > + < /sup > -ATPase and ecto-5 ′-nucleotidase) enzymes associated with neurological conditions. Phenolic-rich extract was prepared using methanol: 1 N HCl (1:1, < em class= " a-plus-plus " > v < /em > / < em class= " a-plus-plus " > v < /em > ) mixture and characterized using high-performance liquid chromatography-diode array detector (HPLC-DAD). In vitro tests were used to investigate the effects of the phenolic extract on AChE, Na < sup class= " a-plus-plus " > + < /sup > /K < sup class= " a-plus-plus " > + < /sup > -ATPase, and ecto-5 ′-nucleotidase activities. Furthermore, the hydroxyl (OH) radical scavenging and Fe < sup class= " a-plus-plus " > 2+ < /sup > -chelating abilities of the extract were investigated. HPLC-DAD analysis revealed the presence of some phenolic acids such as caffeic acid (120.58  mg/g), ferulic acid (76.45 mg/g), gallic acid (17.48 mg/g), chlorogenic acid (16.25 mg/g), and flavonoids such as kaempferol (15.98 mg/g), rutin (51.07 mg/g), quercetin (263.16 mg/g), and quercitrin (89.21 mg/g) in the phenolic extract. The extract significantly inhibited AChE and ecto-5′ -nucleotidase activities in a dose dependent manner with IC < sub class...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research