Development of a novel Loop-Mediated Isothermal Amplification (LAMP) assay for Rickettsia spp.

Development of a novel Loop-Mediated Isothermal Amplification (LAMP) assay for Rickettsia spp. Jpn J Infect Dis. 2016 Jun 30; Authors: Hanaoka N, Matsutani M, Satoh M, Ogawa M, Shirai M, Ando S Abstract We developed a novel loop-mediated isothermal amplification (LAMP) detection method for Rickettsia spp., including Rickettsia prowazekii and Rickettsia typhi. Species-specific LAMP primers were developed for orthologous genes conserved among Rickettsia spp. The selected modified primers could detect all the Rickettsia spp. tested. Thus, the LAMP method was successfully used to detect 100 DNA copies of Rickettsia spp. within approximately 60 min at 63°C. This method may be an excellent tool for the early diagnosis of rickettsiosis in laboratory or in the field. PMID: 27357983 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research