Accuracy of stick-type kit and enzyme-linked immunosorbent assay for the detection of urinary Helicobacter pylori antibodies in a population of Japan Sea region of northern Japan.

Accuracy of stick-type kit and enzyme-linked immunosorbent assay for the detection of urinary Helicobacter pylori antibodies in a population of Japan Sea region of northern Japan. Jpn J Infect Dis. 2016 Jun 30; Authors: Shimoyama T, Sawada Y, Sawada N, Chinda D, Fukuda S Abstract In Japan, both a novel stick-type kit and ELISA kit are available for detection of antibody to Helicobacter pylori (H. pylori) in urine. However, the accuracy of these tests has not been fully examined in northern Japanese populations. Urine samples from 359 subjects were tested using a novel stick-type urinary H. pylori-antibody detection kit (RAPIRUN), while samples from 200 subjects were tested using an ELISA-based test (URINELISA). Prevalence of H. pylori infection was tested by (13)C-urea breath test (UBT) and a monoclonal antibody-based stool antigen test (TPAg). Subjects were considered to have the infection if either UBT or rapid TPAg results were positive. The percentage of positive test results for RAPIRUN and URINELISA was 54.0% and 40.8%, respectively. Sensitivity and specificity were 83.3% and 67.0% for RAPIRUN and 86.5% and 85.8% for URINELISA. Nineteen subjects had cutoff index value of URINERIZA between 0.4 and 0.9, and four of these subjects (21.1%) were found to be infected with H. pylori. The urine-based ELISA is more accurate than the rapid stick-type kit in this series of patients. If negative ELISA results are near the cut-off value, su...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research