Caveolin-1 regulates corneal wound healing by modulating Kir4.1 activity.

Caveolin-1 regulates corneal wound healing by modulating Kir4.1 activity.
Am J Physiol Cell Physiol. 2016 Apr 27;:ajpcell.00023.2016
Authors: Zhang C, Su XT, Bellner L, Lin DH
Abstract
The expression of caveolin-1 (Cav1) in corneal epithelium is associated with regeneration potency. We used Cav1(-/-) mice to study the role of Cav1 in modulating corneal wound healing. Western blot and whole cell patch-clamp were employed to study the effect of Cav1 deletion on Kir4.1 current density in corneas. We found that Ba(2+)-sensitive K(+) currents in primary cultured murine corneal epithelial cells (pMCE) from Cav1(-/-) were dramatically reduced (602pA) in comparison to those from WT (1300pA). As a consequence, membrane potential was elevated in pMCE from Cav1(-/-) comparing to that from WT (-43±7.5 vs -58±4.0 mV, respectively). Western blot showed that either inhibition of Cav1 expression or Ba(2+) incubation stimulated phosphorylation of EGFR. The transwell migration assay showed that Cav1 genetic inactivation accelerated cell migration. The regrowth efficiency of human corneal epithelial cells (HCE) transfected with siRNA-Cav1 or negative control was evaluated by scrape injury assay. With the presence of mitomycin C (10 µg/ml) to avoid the influence of cell proliferation, Cav1 inhibition with siRNA significantly increased migration as compared to control siRNA in HCE. This promoting effect by siRNA-Cav1 could not be further enhanced by c...

Source: Am J Physiol Cell Ph... - April 26, 2016 Category: Cytology Authors: Zhang C, Su XT, Bellner L, Lin DH Tags: Am J Physiol Cell Physiol Source Type: research