Effects of lipopolysacharide on the histomorphology and expression of toll like receptor 4 in the chicken trachea and lung.

Effects of lipopolysacharide on the histomorphology and expression of toll like receptor 4 in the chicken trachea and lung. Avian Pathol. 2016 Mar 24;:1-46 Authors: Ansari AR, Ge XH, Huang HB, Huang XY, Zhao X, Peng KM, Zhong JM, Liu HZ Abstract Endotoxin or lipopolysaccharide (LPS) exposure can cause injury to the respiratory airways and in response, the respiratory epithelia express toll like receptors (TLRs) in many species. However, its role in the innate immunity in the avian respiratory system is poorly understood. The aim of the present study was to evaluate the effects of LPS on the chicken trachea and lung. After intra peritoneal LPS or saline injection, the trachea and lungs were harvested at 0, 12, 36 and 72h (n=6 at each time point) and histopathologically analyzed using hematoxylin and eosin (H&E) and periodic acid-Schiff (PAS) staining, while TLR4 expression was determined by immunohistochemistry and secretory Immunoglobin A (SIgA) levels by ELISA. After LPS stimulation, we observed a remarkable decrease in the number of goblet cells along with obvious disruption and desquamation of the ciliated epithelium in the trachea, blurring of the boundary between pulmonary lobules, narrowed or indistinguishable lumen of the pulmonary atria and leukostasis in the lungs. Following LPS stimulation, TLR4 protein expression was up-regulated in both the trachea and the lungs and was found on the ciliated columnar cells as well as ...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research