Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.

Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.
Monoclon Antib Immunodiagn Immunother. 2016 Mar 16;
Authors: Park YN, Glover RA, Daniels KJ, Soll DR
Abstract
The maltose binding protein (MBP) is a commonly used protein tag. Two monoclonal antibodies (mAbs) were generated against the MBP by immunizing mice with purified 6xHis-tagged MBP (6xHis-MBP). A nontoxic adjuvant cocktail of poly(I:C) and anti-CD40 mAb was used. The two mAbs, 3D7 and 2A1, are demonstrated to be effective in immunoprecipitation, immunoblotting, western blot hybridization, and the ELISA assay. These two mAbs, are available individually or in combination at cost through the Developmental Studies Hybridoma Bank, a nonprofit National Resource created by the National Institutes of Health.
PMID: 26982821 [PubMed - as supplied by publisher]

Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 18, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research