Contribution of Lewis X carbohydrate structure to neuropathogenic murine coronaviral spread.

Contribution of Lewis X carbohydrate structure to neuropathogenic murine coronaviral spread. Jpn J Infect Dis. 2016 Feb 19; Authors: Kakizaki M, Togayachi A, Narimatsu H, Watanabe R Abstract Although Lewis X (Le(x)), a carbohydrate structure, is involved in innate immunity through cell-to-cell and pathogen recognition, Le(x) expression has not been observed in mouse monocytes/macrophages (Mo/Mas). The Mo/Mas that infiltrate the meninges after infection with the neuropathogenic murine coronavirus strain srr7 are an initial target of infection. Furthermore, higher inflammatory responses were observed in gene-manipulated mice lacking α1,3-fucosyltransferase 9, which determines the expression of the Le(x) structure, than those in wild-type mice after infection. We investigated Le(x) expression using CD11b-positive peritoneal exudate cells (PECs), and found that Le(x) is inducible in Mo/Mas after infection with srr7, especially in the syncytial cells during the late phase of infection. The numbers of syncytial cells were reduced after treatment of infected PECs with anti-Le(x) antibody during the late phase of infection. In addition, the antibody treatment induced a marked reduction in numbers of the infected cells at 24 hours post inoculation without changing the infected cell numbers during the initial phase of infection. These data indicate that the Le(x) structure could play a role in syncytial formation and cell-to-cell infection du...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research