Sensitivity and specificity of a latex agglutination test for detection of calf enterotoxigenic Escherichia coli isolates in comparison with PCR

Abstract Latex agglutination test is a very fast, accurate, and specific method that is used to diagnose antigens and antibodies which can be carried out in any place without need for any specific devices. In the present study, a single chain (scFv) recombinant monoclonal anti-K99 antibody was used in designing and producing a latex agglutination kit for the detection of enterotoxigenic Escherichia coli of calves colibacillosis. The purpose of this research was to primarily evaluate the sensitivity and specificity of the mentioned latex agglutination kit in the detection of the considered bacteria and to compare it with the results of PCR method. To carry out latex agglutination method, carboxyl latex particles were covered by the recombinant monoclonal antibodies of anti-K99 scFv. Then, these latex particles were mixed with the bacterial suspension purified from the diarrhea samples of the calves on the black cards of kit to observe agglutination. Sensitivity and specificity of the latex agglutination kit in the diagnosis of this colonization factor was evaluated and compared with the results of PCR test. Comparison of the PCR test results with those of the kit indicated that, under the laboratory conditions and on the purified bacteria, sensitivity and specificity levels of the latex agglutination kit were 100 and 96 %, respectively. Primary evaluation showed that the new latex agglutination kit was able to quickly diagnose the K99+ bacteria and can be used fo...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

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Publication date: Available online 20 September 2020Source: Journal of Global Antimicrobial ResistanceAuthor(s): Yanfei Wang, Haiyang Liu, Qian Wang, Xiaoxing Du, Yunsong Yu, Yan Jiang
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Conclusion: ZnO nanocoating may improve the antibacterial effects of NiTi wires and reduce the frictional resistance. Coating may be implanted in orthodontic practice for faster and safer treatment.RESUMO Objetivos: Avaliar o efeito do nanorrevestimento de óxido de zinco (ZnO) sobre as propriedades mecânicas e propriedades antibacterianas de fios ortodônticos de NiTi. Métodos: Fios 0,016 ” x 0,022 ” de NiTi foram revestidos com nanopart ículas de ZnO por meio de um método de deposição eletroquímica com um sistema de três eletrodos a 0,1M Zn(NO3)...
Source: Dental Press Journal of Orthodontics - Category: Dentistry Source Type: research
Ionizing radiation (IR) is lethal to most organisms at high doses, damaging every cellular macromolecule via induction of reactive oxygen species (ROS). Utilizing experimental evolution and continuing previous work, we have generated the most IR-resistant Escherichia coli populations developed to date. After 100 cycles of selection, the dose required to kill 99% the four replicate populations (IR9-100, IR10-100, IR11-100, and IR12-100) has increased from 750 Gy to approximately 3,000 Gy. Fitness trade-offs, specialization, and clonal interference are evident. Long-lived competing sub-populations are present in three of the...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
kungo Public schools in most rural areas of South Africa depend on untreated groundwater due to unreliable water supply by the municipalities. This has the potential to cause water-related health problems to school children. Temperature, pH, and electrical conductivity (EC) were measured in situ. Chemical (fluoride, chloride, sulphate, phosphate, nitrate, magnesium, calcium, sodium, and potassium) and microbial (Escherichia coliform (E. coli), Salmonella typhimurium, and Shigella flexneri) water quality parameters were analysed in groundwater samples from 10 public schools in Vhuronga 1 to determine suitability for use...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
Faraday Discuss., 2020, Accepted Manuscript DOI: 10.1039/D0FD00046A, PaperJosefine Eils ø Nielsen, Sylvain Prevost, jenssen havard, Reidar Lund The mechanism of action of antimicrobial peptides (AMPs) has been debated over many years, and various models have been proposed. In this work we combine small angle X-ray/neutron scattering (SAXS/SANS)... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Faraday Discuss. latest articles - Category: Chemistry Authors: Source Type: research
(Cornell University) Cornell University scientists have engineered a key plant enzyme and introduced it in Escherichia coli bacteria in order to create an optimal experimental environment for studying how to speed up photosynthesis, a holy grail for improving crop yields.
Source: EurekAlert! - Biology - Category: Biology Source Type: news
Publication date: Available online 19 September 2020Source: Journal of BiotechnologyAuthor(s): J. Porter Hunt, R. Jordan Barnett, Hannah Robinson, Mehran Soltani, J. Andrew D. Nelson, Bradley C. Bundy
Source: Journal of Biotechnology - Category: Biotechnology Source Type: research
Advances in DNA sequencing have revolutionized our ability to read genomes. However, even in the most well-studied of organisms, the bacteriumEscherichia coli, for ≈ 65% of promoters we remain ignorant of their regulation. Until we crack this regulatory Rosetta Stone, efforts to read and write genomes will remain haphazard. We introduce a new method, Reg-Seq, that links massively-parallel reporter assays with mass spectrometry to produce a base pair resoluti on dissection of more than 100E. colipromoters in 12 growth conditions. We demonstrate that the method recapitulates known regulatory information. Then, we exami...
Source: eLife - Category: Biomedical Science Tags: Physics of Living Systems Source Type: research
Microbial Drug Resistance, Ahead of Print.
Source: Microbial Drug Resistance - Category: Microbiology Authors: Source Type: research
AbstractOtitis externa is a common condition encountered in the ENT outdoors. For long, it was thought to be of fungal etiology but after World War II, bacterial pathogens were found to be most commonly involved.Pseudomonas has been described as the most common causative organism in the literature. This prospective study aimed to study the microbiological profile and antibiotic sensitivity of 100 patients of otitis externa. 100 diagnosed cases of otitis externa were included in the study after informed consent. Swabs were taken from the external acoustic canal maintaining asepsis. The swabs were analysed using microscopy, ...
Source: Indian Journal of Otolaryngology and Head and Neck Surgery - Category: ENT & OMF Source Type: research
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