RNA interference screening of interferon-stimulated genes with antiviral activities against classical swine fever virus using a reporter virus

In this study, to conduct the screening of anti-CSFV ISGs, twenty-one ISGs reported previously were individually knocked down using small interfering RNAs (siRNAs) followed by infection with a reporter CSFV expressing Renilla luciferase (Rluc). As a result, four novel anti-CSFV ISGs were identified, including natural-resistance-associated macrophage protein 1 (NRAMP1), cytosolic 5′-nucleotidase III A (NT5C3A), chemokine C-X-C motif ligand 10 (CXCL10), and 2′-5′-oligoadenylate synthetase 1 (OAS1), which were further verified to exhibit antiviral activities against wild-type CSFV. We conclude that the reporter virus is a useful tool for efficient screening anti-CSFV ISGs.
Source: Antiviral Therapy - Category: Virology Source Type: research