CRISPR-Cas9 and Sleeping Beauty Transposons

Neuromics-B-Mogen ApproachThe CRISPR-Cas9 gene editing approach is now officially considered a revolution. This is confirmed by the increasing frequency of this word being used across the web in describing this technique. In fact, it is now appearing in conference titles. see: Genome Engineering: The CRISPR/Cas Revolution 2016COLD SPRING HARBOR - 17 AUG 2016.We have joined the revolution with our new Sleeping Beauty TransposonTM Systems.Figure: Sleeping Beauty TransposonTM Systems Gene Integration Process. (A) A depiction of Sleeping Beauty transposon plasmid and Sleeping Beauty transposase enzyme active in cellular nuclei. (B) Transposase enzyme binds to Sleeping Beauty-specific IR/DR sites. (C) Transposase enzyme excises transposon sequence from transposon vector. (D) Transposase enzyme stably integrates transposon sequence at TA site in host cell genome.Features Include:Simple-"Cut and Paste" Integration and Editing. Check out our Sleeping Beauty Transposon System Users Manual Potent-100% Stable with No Off target effects with the safest transposon insertion profile. Fast- Transfect with our transposase + Sleeping Beauty Transposon Reporter Series Vectors. Cost Effective- Starting at $329.If you would like us to engineer custom Transposons or gene editing on your cells, please conatact me directly and we will work together to formulate a Statement of Work to your specifications. Pete Shuster, CEO and Owner, Neuromics, [e-mail] or cell phone: 61...

Source: Neuromics - January 3, 2016 Category: Neuroscience Tags: cas9 CRISP CRISP-Cas9 gene editing gene expression analysis gene integration Sleeping Beauty. transposase transposons Source Type: news