Pentosan Polysulfate Preserves Renal Microvascular P2X1 Receptor Reactivity and Autoregulatory Behavior in DOCA-salt Hypertensive Rats.

Pentosan Polysulfate Preserves Renal Microvascular P2X1 Receptor Reactivity and Autoregulatory Behavior in DOCA-salt Hypertensive Rats. Am J Physiol Renal Physiol. 2015 Dec 23;:ajprenal.00110.2015 Authors: Guan Z, Singletary ST, Cha H, Van Beusecum JP, Cook AK, Pollock JS, Pollock DM, Inscho EW Abstract Inflammation contributes to Ang II-associated impairment of renal autoregulation and microvascular P2X1 receptor signaling, but its role in renal autoregulation in mineralocorticoid-induced hypertension is unknown. Autoregulatory behavior was assessed using the blood-perfused juxtamedullary nephron preparation. Hypertension was induced in uninephrectomized-control rats (UNx) by subcutaneous implantation of a deoxycorticosterone acetate pellet plus 1% NaCl to drink (DOCA-salt) for 3 weeks. DOCA-salt rats developed hypertension that was unaltered by anti-inflammatory treatment with pentosan polysulfate (DOCA-salt+PPS) but was suppressed with "triple therapy" (hydrochlorothiazide, hydralazine and reserpine; DOCA-salt+TTx). Baseline arteriolar diameters were similar across all groups. UNx rats exhibited pressure-dependent vasoconstriction with diameter declining to 69±2% of control at 170 mmHg, indicating intact autoregulation. DOCA-salt treatment significantly blunted this pressure-mediated vasoconstriction. Diameter remained between 91±4 and 98±3% of control over 65-170 mmHg, indicating impaired autoregulation. In contrast, pressure-...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research