Inhibition potential of Moringa oleifera Lam. on drug metabolizing enzymes
Title: Inhibition potential of Moringa oleifera Lam. on drug metabolizing enzymes
Authors: Ahmmed, SK Milan; Mukherjee, Pulok K; Bahadur, Shiv; Kar, Amit; Al-Dhabi, Naif Abdullah; Duraipandiyan, V
Abstract: The leaves of Moringa oleifera
Lam. (Family:
Moringaceae) has
been traditionally used in Indian system of medicine (ISM) for its wide
spectrum of pharmacological effects. The aim of this study was to evaluate the
inhibitory effect of standardized M.
oleifera extract and its biomolecule chlorogenic acid on drug metabolizing
enzymes (CYP450) which may cause interaction. The amount of chlorogenic acid
was determined by using high performance thin layer chromatography (HPTLC).
CYP-CO complex assay and fluorescence assay were followed for this study by
using rat liver micro some (RLM) and two key isozymes CYP3A4 and CYP2D6. The
hydro-alcoholic extract of M. oleifera
contains 2.99 %
(w/w)
of chlorogenic acid by HPTLC analysis. The CYP-CO complex assay showed 23.45 ± 0.78
%
inhibition on RLM. The fluorescence study revealed that both the extract and
chlorogenic acid (IC50: 127.36 ± 2.98, 146.50 ± 3.46 µg/mL and 133.73 ± 1.79,
159.22 ± 3.53 µg/mL) had some inhibition with both isozymes CYP3A4 and CYP2D6 but very less in
comparison to their respective positive inhibitors ketoconazole and quinidine.
Thus it may be concluded that M. oleifera
extract has very less potential to inhibit the CYP isozymes, so traditional use
of this plant may be safe.
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Source: Indian Journal of Traditional Knowledge - Category: Science Authors: search Source Type: research
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