Genetic differences in ChTLR15 gene polymorphism and expression involved in Salmonella enterica natural and artificial infection respectively of Chinese native chicken breeds, with a focus on sexual dimorphism.

In this study, the correlation between ChTLR15 SNPs and the natural infection status of salmonella in hens, as well as the genetic and sex-specific effects on ChTLR15 mRNA expression in heterophils and the spleen during the acute Salmonella enterica serovar Enteritidis (S. Enteritidis) infection period (from 1 to 10 days after infection), were studied in two genetically distinct Chinese breeds (Qingyuan Partridge chickens and Baier chickens). Three SNPs (G168A, C726T and A1166G) in a single exon of ChTLR15 were identified in the two breeds, but only C726T showed a significant association with salmonella infection. Compared with laying-type Baier chicks, meat-type Qingyuan chicks showed a higher tolerance for capture stress and S. Enteritidis infection, as measured respectively by the modified body weight of chicks in the control group and in the infection group. Meanwhile, ChTLR15 down-regulation in heterophils and up-regulation in spleen was involved in the response to pathogenic S. Enteritidis colonization during the acute infection period. These significant genetic effects in females led to greater differences in both innate and adaptive immune responses than those exhibited in males. These results suggest that genetics, time and sex play important roles in the modulation of ChTLR15 mRNA level elicited by the S. Enteritidis -mediated immune response differentially in the two genetically distinct breeds, with a focus on sexual dimorphism. PMID: 26488442 [PubMed - as...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research