Bacteriocin producing probiotic Lactobacillus plantarum inhibit adhesion of Staphylococcus aureus to extracellular matrix (ECM): Quantitative insight and implications in antibacterial therapy.

Bacteriocin producing probiotic Lactobacillus plantarum inhibit adhesion of Staphylococcus aureus to extracellular matrix (ECM): Quantitative insight and implications in antibacterial therapy.
J Med Microbiol. 2015 Oct 6;
Authors: Mukherjee S, Ramesh A
Abstract
In the present study, the adhesion of bacteriocin producing probiotic strains of Lactobacillus plantarum onto extracellular matrix (ECM) proteins such as collagen and mucin and their potential to prevent pathogen invasion onto ECM was ascertained. Fluorescence-based in vitro assays indicated that the L. plantarum strains CRA21, CRA38 and CRA52 displayed considerable adhesion onto ECM molecules, which was comparable to the probiotic L. rhamnosus GG. Flow cytometry-based quantitative assessment of the adhesion potential suggested that L. plantarum CRA21 exhibited superior adhesion onto ECM as compared to other LAB strains. Furthermore, fluorescence-based assays suggested that the highest inhibition of S. aureus adhesion onto collagen and mucin by bacteriocin producing L. plantarum strains was observed in the exclusion mode as compared to competition and displacement mode. This observation was supported by the higher binding affinity (kd) for ECM exhibited by the L. plantarum strains as compared to S. aureus. Interestingly, a crude plantaricin A extract from the food isolates of L. plantarum displayed potent antibacterial activity on ECM-adhered S. aureus cells. It is envisaged t...

Source: Journal of Medical Microbiology - October 6, 2015 Category: Microbiology Authors: Mukherjee S, Ramesh A Tags: J Med Microbiol Source Type: research