Effect of honokiol on exotoxin proteins listeriolysin O and p60 secreted by Listeria monocytogenes.

Effect of honokiol on exotoxin proteins listeriolysin O and p60 secreted by Listeria monocytogenes. J Med Microbiol. 2015 Oct 6; Authors: Meng R, Zhao Z, Guo N, Liu Z, Zhao X, Li W, Li X, Shi C, Nie D, Wang W, Liu T, Ma W, Yu L, Li J Abstract Listeria monocytogenes (L. monocytogenes) is considered one of the most important foodborne pathogens. The virulence-related proteins listeriolysin O (LLO) and p60 are critical factors involved in Listeria pathogenesis. In the present study, we investigated the effect of honokiol on LLO and p60 secreted from L. monocytogenes. A listeriolysin assay was used to investigate hemolytic activities of L. monocytogenes exposed to honokiol, and the secretion of LLO and p60 was detected by immunoblot analysis. Additionally, the influence of honokiol on the transcription of LLO and p60 encoded genes hly and iap, respectively, was analyzed by real-time RT-PCR. Tumor necrosis factor (TNF) release assays were performed to elucidate the biological relevance of changes in LLO and p60 secretion induced by honokiol. According to the data, honokiol showed good anti-L. monocytogenes activity, with minimum inhibitory concentrations of 8-16 µg/ml, and the secretion of LLO and p60 was decreased by honokiol. Meanwhile, the transcription of hly and iap were inhibited by honokiol. Our results indicated that TNF-α production by RAW264.7 cells stimulated with L. monocytogenes supernatants was inhibited by honokiol. Based...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research