Retinal Microvascular Endothelial Cells & Diabetic Retinopathy

In this study, the authors show that human retinal microvascular endothelial cells exposed to high levels of glucose regulate miR-200b repression through histone methylation and that inhibition of PRC2 increases miR-200b while reducing VEGF: Michael Anthony Ruiz, Biao Feng, and Subrata Chakrabarti. Polycomb Repressive Complex 2 Regulates MiR-200b in Retinal Endothelial Cells: Potential Relevance in Diabetic Retinopathy. PLoS One. 2015; 10(4): e0123987. Published online 2015 Apr 17. doi: 10.1371/journal.pone.0123987...To investigate PRC2 and miR-200b regulation in the context of diabetic retinopathy, the major cell type used for investigation was the human retinal microvascular endothelial cell (HRMECs,Neuromics/ Olaf Pharmaceuticals, Worchester, MA, Cat# HEC09)...Figure: High levels of glucose alter VEGF and miR-200b expression in HRMECs. A: HRMECs exposed to various concentrations of D-glucose for 24 hours exhibited differential mRNA levels of VEGF. Compared to 5mM D-glucose, VEGF expression was significantly increased at 15mM and 25mM D-glucose concentrations, with no change at 20mM L-glucose. B: Measured by WST-1 assay, HRMECs exposed to increasing concentrations of D-glucose for 24 hours exhibited decreased cell viability at 25mM, 50mM and 100mM compared to 5mM. C: HRMECs exposed to 25mM (high glucose; HG) glucose for 24 and 48 hours demonstrated significantly increased VEGF mRNA compared to 5mM (normal glucose; NG). These differences were not observed at time points earl...
Source: Neuromics - Category: Neuroscience Tags: Glucose HRMECS Human Endothelial Cells Human Retinal Microvascular Cells VEGF Source Type: news