Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of DNA in Blood Samples from Chagas Disease Patients
An international study was performed by 26 experienced PCR laboratories from 14 countries to assess the performance of duplex quantitative real-time PCR (qPCR) strategies on the basis of TaqMan probes for detection and quantification of parasitic loads in peripheral blood samples from Chagas disease patients. Two methods were studied: Satellite DNA (SatDNA) qPCR and kinetoplastid DNA (kDNA) qPCR. Both methods included an internal amplification control. Reportable range, analytical sensitivity, limits of detection and quantification, and precision were estimated according to international guidelines.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Juan Carlos RamÃrez, Carolina Inés Cura, Otacilio da Cruz Moreira, Eliane Lages-Silva, Natalia Juiz, Elsa Velázquez, Juan David RamÃrez, Anahà Alberti, Paula Pavia, MarÃa Delmans Flores-Chávez, Arturo Muñoz-Calderón, Deyanira Pérez-Morales, Josà Tags: Regular article Source Type: research