Impaired unfolded protein response in the degeneration of cochlea cells in a mouse model of age-related hearing loss.

In this study, we measured the expression levels of GRP78 and ubiquitinated proteins in the cochleae of young C57BL/6 mice and aged mice to assess the capacity of the UPR. The lower expression of GRP78 and the increased number of ubiquitinated proteins observed in the cochleae of aged mice suggested that the capacity of the UPR was impaired and that the cell death pathway was activated. We found a markedly increased expression of the ER-related pro-apoptotic factor C/EBP homologous protein (CHOP) in the cochleae of aged mice, whereas the level of cleaved caspase-12 did not differ between the two groups. In addition, the cleavage of caspase-9, caspase-3 and poly [ADP-ribose] polymerase 1 was significantly increased in the aged cochleae, suggesting the activation of apoptosis in the cochleae resulting from the cross-talk between the ER and mitochondria through CHOP. These results indicated that impaired UPR in the cochleae of aged C57BL/6 mice resulting in ER stress may lead to apoptosis that is dependent on the mitochondrial pathway and that ER stress induced apoptosis may not be mediated by caspase-12. PMID: 26173054 [PubMed - as supplied by publisher]
Source: Experimental Gerontology - Category: Geriatrics Authors: Tags: Exp Gerontol Source Type: research