Integrating recombinase polymerase amplification with CRISPR/Cas9-initiated nicking-rolling circle amplification in < em > Staphylococcus aureus < /em > assay

Chem Commun (Camb). 2024 Apr 26. doi: 10.1039/d4cc00238e. Online ahead of print.ABSTRACTWe integrate recombinase polymerase amplification (RPA) with CRISPR/Cas9-initiated nicking rolling circle amplification (CRISPR/Cas9-nRCA) for detecting Staphylococcus aureus. This approach utilizes a unique dimeric G-triplex structure, demonstrating firstly enhanced ThT fluorescence for target detection. The proof-of-concept study introduces a new avenue for integrating isothermal amplifications with CRISPR/Cas9 in the fields of pathogen detection and disease diagnosis.PMID:38666524 | DOI:10.1039/d4cc00238e
Source: Chemical Communications - Category: Chemistry Authors: Source Type: research