Tuning Structural Characteristics of Corn Stover Through Ammonium and Sodium Sulfite (ASS) Pretreatment for Enhanced Enzymatic Hydrolysis

In this study, we have updated the AFEX pretreatment method; ammonia and sodium sulfite (ASS) can be used to produce a more digestible substrate. The results demonstrate that ASS-pretreated corn stover (CS) yields a higher fermentable sugar yield compared with AFEX pretreatment, even at lower enzyme loadings. Specifically, at an enzyme loading of 12 mg protein/g glucan, ASS-CS achieved 88.8% glucose and 80.6% xylose yield. Characterization analysis reveals that lignin underwent sulfonation during ASS pretreatment. This modification results in a more negative zeta potential for ASS-CS, indicating a reduction in nonproductive adsorption between lignin and cellulase through increased electrostatic repulsion.PMID:38668842 | DOI:10.1007/s12010-024-04964-w
Source: Applied Biochemistry and Biotechnology - Category: Biochemistry Authors: Source Type: research