Ecotoxicological strategies employing biochemical markers and organisms to monitor the efficacy of malathion photolysis treatment

The objective of this study was to assess the photolysis-mediated degradation of malathion in standard and commercial formulations, and to determine the toxicity of these degraded formulations. Degradation tests were carried out with 500 μg L-1 of malathion and repeated three times. The initial and residual toxicity was assessed by using Lactuca sativa seeds for phytotoxicity, Stegomyia aegypti larvae for acute toxicity, and Stegomyia aegypti mosquitoes (cultivated from the larval stage until emergence as mosquitoes) to evaluate the biochemical markers of sublethal concentrations. For the standard formulations the photolytic process efficiently reduced the initial concentration of malathion to levels below the regulatory limits however, the formation of byproducts was revealed by chromatography, which allowed for a more complete proposal of photolytic-mediated malathion degradation route. The degraded formulations inhibited the growth of L. sativa seeds, while only the untreated formulations showed larvicidal activity and mortality. Both formulations slightly inhibited acetylcholinesterase activity in S. aegypti mosquitoes, while the standard formulation decreased and the commercial formulation increased glutathione S-transferase activity. However, there were no significant differences for superoxide dismutase, esterase-α, esterase-β and lipid peroxidation. These findings indicate that in the absence of the target compound, the presence of byproducts can alter the enzymati...

Source: Chemosphere - April 24, 2024 Category: Chemistry Authors: Rafaela Imoski La ís Jarenko da Cruz Angela Maria Palacio-Cort és Ana Marta Schafaschek Eric Schwamberger Pamella Regina Mariotti Andre Luis Bichibichi Borges Fernando Rodrigues-Silva Lizi ê Daniela Tentler Prola Mario Ant ônio Navarro da Silva Adrian Source Type: research