Viruses, Vol. 16, Pages 646: PCV2 Induced Endothelial Derived IL-8 Affects MoDCs Maturation Mainly via NF- & kappa;B Signaling Pathway

Viruses, Vol. 16, Pages 646: PCV2 Induced Endothelial Derived IL-8 Affects MoDCs Maturation Mainly via NF-κB Signaling Pathway Viruses doi: 10.3390/v16040646 Authors: Mengyu Zhang Weicheng Xu Ning Yang Zhuowei Li Shuanghai Zhou Xuewei Liu Jianfang Wang Huanrong Li Porcine circovirus type 2 (PCV2) infection can cause immunosuppressive diseases in pigs. Vascular endothelial cells (VECs), as the target cells for PCV2, play an important role in the immune response and inflammatory regulation. Endothelial IL-8, which is produced by porcine hip artery endothelial cells (PIECs) infected with PCV2, can inhibit the maturation of monocyte-derived dendritic cells (MoDCs). Here, we established a co-culture system of MoDCs and different groups of PIECs to further investigate the PCV2-induced endothelial IL-8 signaling pathway that drives the inhibition of MoDC maturation. The differentially expressed genes related to MoDC maturation were mainly enriched in the NF-κB and JAK2-STAT3 signaling pathways. Both the NF-κB related factor RELA and JAK2-STAT3 signaling pathway related factors (IL2RA, JAK, STAT2, STAT5, IL23A, IL7, etc.) decreased significantly in the IL-8 up-regulated group, and increased significantly in the down-regulated group. The expression of NF-κB p65 in the IL-8 up-regulated group was reduced significantly, and the expression of IκBα was increased significantly. Nuclear tra...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research