eCRUIS captures RNA-protein interaction in vitro and in vivo

Exp Cell Res. 2024 Apr 15:114051. doi: 10.1016/j.yexcr.2024.114051. Online ahead of print.ABSTRACTAs an information bridge between DNA and protein, RNA regulates cellular processes and gene expression in various ways. From synthesis to degradation, RNA is associated with a range of RNA-binding proteins. Therefore, it is necessary to develop innovative methods to study the interactions between RNA and proteins. Previously, we developed an RNA-centric method, called CRISPR-based RNA-United Interacting System (CRUIS), to capture RNA-protein interaction in cells. On this basis, here we develop an enhanced CRUIS (eCRUIS) by combining the power of dCas13d and the engineered promiscuous ligase TurboID. The current version allows us to label RNA-binding proteins on the target RNA within 30 minutes, potentially for in vivo use. By introducing bait-assay with exogenous RNA, we confirm that eCRUIS can effectively label RNA-binding proteins on bait RNA in a short time. eCRUIS provides a broader range of in vitro and in vivo applications.PMID:38631547 | DOI:10.1016/j.yexcr.2024.114051

Source: Experimental Cell Research - April 17, 2024 Category: Cytology Authors: Ziheng Zhang Yuanbing Zhang Ji-Long Liu Source Type: research