Molecular architecture of the actin cytoskeleton: From single cells to whole organisms using cryo-electron tomography

Curr Opin Cell Biol. 2024 Apr 11;88:102356. doi: 10.1016/j.ceb.2024.102356. Online ahead of print.ABSTRACTCryo-electron tomography (cryo-ET) has begun to provide intricate views of cellular architecture at unprecedented resolutions. Considerable efforts are being made to further optimize and automate the cryo-ET workflow, from sample preparation to data acquisition and analysis, to enable visual proteomics inside of cells. Here, we will discuss the latest advances in cryo-ET that go hand in hand with their application to the actin cytoskeleton. The development of deep learning tools for automated annotation of tomographic reconstructions and the serial lift-out sample preparation procedure will soon make it possible to perform high-resolution structural biology in a whole new range of samples, from multicellular organisms to organoids and tissues.PMID:38608425 | DOI:10.1016/j.ceb.2024.102356
Source: Current Opinion in Cell Biology - Category: Cytology Authors: Source Type: research