Genomic characterization of ST38 NDM-5-producing < em > Escherichia coli < /em > isolates from an outbreak in the Czech Republic

Antimicrob Agents Chemother. 2024 Apr 16:e0013324. doi: 10.1128/aac.00133-24. Online ahead of print.ABSTRACTA 2-year national genomic screening in the Czech Republic identified a notable prevalence of the New Delhi metallo-β-lactamase 5 (NDM-5)-producing Escherichia coli sequence type 38 (ST38) in the city of Brno. Forty-two ST38 E. coli isolates harbored the blaNDM-5 gene on the chromosome. Virulence factors confirmed the persistence of these isolates through biofilm formation. Single Nucleotide Polymorphisms (SNPs)-based phylogeny and CRISPR assay typing showed minimal genomic variations, implying a clonally driven outbreak. Results suggest that this high-risk clone may impose a nationwide problem.PMID:38624228 | DOI:10.1128/aac.00133-24

Source: Antimicrobial Agents and Chemotherapy - April 16, 2024 Category: Microbiology Authors: Katerina Chudejova Tsolaire Sourenian Jana Palkovicova Katarina Stredanska Lucie Nechutna Katerina Vlkova Vendula Studentova Working Group for Monitoring of Antibiotic Resistance Jaroslav Hrabak Costas C Papagiannitsis Monika Dolejska Ibrahim Bitar Workin Source Type: research