Alarin potentiates ongoing epileptiform activity in rat brain slices: an in vitro electrophysiological study

In this study, we investigated if alarin induced any synchronous discharges or epileptiform activity, and if it had any effect on already initiated epileptiform discharges. We used thick acute horizontal hippocampal slices obtained from 30‑ to 35‑day‑old rats. Extracellular field potential recordings were evaluated in the CA1 region of the hippocampus. Our data demonstrated that, alarin application did not result in any epileptiform activity or abnormal discharges. 4‑aminopyridine was applied to induce epileptiform activity in the slices. We found that alarin increased the frequency of interictal‑like events and the mean power of local field potentials in the CA1 region of the hippocampus, which was induced by 4‑aminopyridine. These results demonstrated for the first time that alarin has a modulatory effect on synchronized neuronal discharges and showed the contribution of the neuropeptide alarin to epilepsy‑like conditions.PMID:38587326 | DOI:10.55782/ane-2024-2520

Source: Acta Neurobiologiae Experimentalis - April 8, 2024 Category: Neurology Authors: Ömer Faruk Kalkan Hilal Öztürk Zafer Şahin Harun Ba şoğlu Selcen Aydin Abidin İsmail Abidin Source Type: research