Annexin A3: a newly identified player in store ‑operated calcium entry
Acta Neurobiol Exp (Wars). 2021;81(4):307-313.ABSTRACTStore‑operated calcium entry (SOCE) is important for refilling endoplasmic reticulum (ER) Ca2+ stores and Ca2+ signaling. Extracellular Ca2+ is conducted by highly Ca2+‑selective Orai channels that are activated by stromal interaction molecule proteins (STIMs), which are sensitive ER Ca2+ sensors. We found an approximately five‑fold increase in annexin A3a (anxa3a) expression levels in stim2b knockout zebrafish. The present study investigated whether annexin A3 protein is involved in SOCE. We used Ca2+ imaging and electrophysiological recordings to determine the e...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Agnieszka Dyrda Jacek Kuznicki Lukasz Majewski Source Type: research

Neurotrophic factors in Alzheimer's disease: pathogenesis and therapy
Acta Neurobiol Exp (Wars). 2021;81(4):314-327.ABSTRACTAlzheimer's disease (AD) is a common neurodegenerative disease with a prevalence estimated to reach 115 million by 2050. It is characterized by abnormal extracellular accumulation of amyloid‑beta (Aβ) peptide and intracellular neurofibrillary tangles (NFTs) that result in neuro‑inflammation, synaptic dysfunction, neurotransmitter imbalance, neuronal loss, and dendritic changes. A hypothesis of neurotrophic factor (NTF) involvement in neurodegenerative diseases and their potential as a therapeutic tool has emerged. There are wide information gaps on this topic. ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Joachim Hr L übke Faezeh Idoon Mina Mohasel-Roodi Fatemeh Alipour Javad Hami Alireza Ehteshampour Hamideh Mostafaee Akram Sadeghi Source Type: research

Morinda citrifolia Linn. fruit extract mitigates heroin seeking behavior in mice
This study demonstrates that MMC attenuated heroin seeking at different phases of drug self‑administration in a mouse modified runway model.PMID:35014982 (Source: Acta Neurobiologiae Experimentalis)
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Vijayapandi Pandy Yasmin Khan Durga Priyanka Yarlagadda Sri Pravallika Tatinada Source Type: research

Combinations of polyphenols disaggregate A β1‑42 by passing through in vitro blood brain barrier developed by endothelium, astrocyte, and differentiated SH‑SY5Y cells
Acta Neurobiol Exp (Wars). 2021;81(4):335-349.ABSTRACTDisaggregation of amyloid βeta (Aβ) is considered as one of the promising therapeutic strategies for Alzheimer's disease. Polyphenols are promising molecules for the disaggregation of Aβ. However, in order to find a potential therapeutic candidate, the in vitro analyses need to be performed on a model that mimics the blood‑brain barrier (BBB) as much as possible. Therefore, we aimed to establish an in vitro BBB representative transwell system by using differentiated human neuroblastoma (SH‑SY5Y), cerebral microvascular endothelial, and astrocyte cells...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Belgin Sert Serdar Tu ğba Erkmen Semra Ko çtürk Source Type: research

Synergistic analgesic effect of morphine and tramadol in non ‑sensitized and morphine‑sensitized mice: an isobolographic study
Acta Neurobiol Exp (Wars). 2021;81(4):350-361.ABSTRACTMorphine and tramadol are the opioid analgesic drugs acting via activation of μ‑opioid receptors. It is important to understand which mechanism (synergistic or additive anti‑nociceptive activity) induced potent anti‑nociceptive effect by co‑administration of morphine and tramadol. Identification of new strategies that can potentiate analgesic effects of opioids will be good therapeutic approaches for pain relief. To this aim, male mice were cannulated in the left ventricle by a stereotaxic instrument. A tail‑flick test was used to record the pain threshold....
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Fatemeh Khakpai Fatemeh Nedaei Faezeh Shahini Mohammad-Reza Zarrindast Source Type: research

Knockdown of NEAT1 induced microglial M2 polarization via miR ‑374a‑5p/NFAT5 axis to inhibit inflammatory response caused by OGD/R
Acta Neurobiol Exp (Wars). 2021;81(4):362-374.ABSTRACTThe long non‑coding RNAs (lncRNAs) have been important regulators for the progression of ischemic‑induced stroke. We aim to study the role of the lncRNA nuclear enriched abundant transcript 1 (NEAT1) in oxygen and glucose deprivation/reoxygenation (OGD/R) treated microglia. OGD/R injury of CHME5 cells was used as an in vitro stroke model. qRT‑PCR analysis was performed to examine NEAT1, miR‑374a‑5p, nuclear factor of activated T cells 5 (NFAT5) and cytokines. Western blot assay detected protein levels of NFAT5 and microglia markers. The concentration of cytoki...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Xiao-Wen Lian Bin Luo Source Type: research

Lipoic acid alleviates LPS ‑evoked PC12 cell damage by targeting p53 and inactivating the NF‑κB pathway
Acta Neurobiol Exp (Wars). 2021;81(4):375-385.ABSTRACTLipoic acid (LA) exerts several beneficial effects including anti‑inflammatory and antioxidant activity. This research aims to explore the function and mechanisms of LA on lipopolysaccharide (LPS)‑induced PC12 cells. PC12 cells stimulated by LPS were used to mimic an in vitro inflammatory model of Parkinson's disease. Cell toxicity was determined by a cell counting kit‑8 assay after various treatments. The concentrations of tumor necrosis factor (TNF-α), interleukin (IL)‑1β and IL‑6 were analyzed by an ELISA kit. The effects of LA on cell apoptosis ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Jieping Mao Hua Gao Wen Bai Haibo Zeng Yafang Ren Yu Liu Xinling Yang Source Type: research

Greater occipital nerve block efficacy in COVID ‑19 associated headache: a preliminary study
Acta Neurobiol Exp (Wars). 2021;81(4):386-392.ABSTRACTCOVID‑19 is an infection caused by the new coronavirus SARS‑CoV‑2. Headache is one of the most common neurological findings. In the treatment of various chronic headaches, the greater occipital nerve (GON) block is often used as a safe and effective method. The aim of our study was to investigate the effectiveness of the GON block in the treatment of headaches observed in COVID‑19 patients. Between March and May 2020, 27 patients (with laboratory‑confirmed 2019‑nCoV infection by next‑generation sequencing confirmation of real‑time PCR) that had moderate ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Ömer Karadaş Hakan Levent G ül Bilgin Öztürk Ali Riza Sonkaya Ak çay Övünç Özön Javid Shafiyev Ender Sir Source Type: research

Identification of traditional Japanese Kampo medicines and crude drugs that upregulate brain ‑derived neurotrophic factor in human peripheral cells
Acta Neurobiol Exp (Wars). 2021;81(4):393-404.ABSTRACTThe neurotrophic hypothesis of depression, which suggests that decreased hippocampal brain‑derived neurotrophic factor (BDNF) levels cause depression, has become increasingly popular. BDNF, a member of the neurotrophin family, promotes neuronal differentiation and survival. BDNF is synthesized in various peripheral tissues, as well as in the brain. Considering that peripheral BDNF can be transported into the brain across the blood‑brain barrier, substances with the ability to upregulate BDNF activity in peripheral tissues may be useful in the management of depressio...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Kensuke Nakajima Shinya Okubo Shigeru Oiso Source Type: research

Annexin A3: a newly identified player in store ‑operated calcium entry
Acta Neurobiol Exp (Wars). 2021;81(4):307-313.ABSTRACTStore‑operated calcium entry (SOCE) is important for refilling endoplasmic reticulum (ER) Ca2+ stores and Ca2+ signaling. Extracellular Ca2+ is conducted by highly Ca2+‑selective Orai channels that are activated by stromal interaction molecule proteins (STIMs), which are sensitive ER Ca2+ sensors. We found an approximately five‑fold increase in annexin A3a (anxa3a) expression levels in stim2b knockout zebrafish. The present study investigated whether annexin A3 protein is involved in SOCE. We used Ca2+ imaging and electrophysiological recordings to determine the e...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Agnieszka Dyrda Jacek Kuznicki Lukasz Majewski Source Type: research

Neurotrophic factors in Alzheimer's disease: pathogenesis and therapy
Acta Neurobiol Exp (Wars). 2021;81(4):314-327.ABSTRACTAlzheimer's disease (AD) is a common neurodegenerative disease with a prevalence estimated to reach 115 million by 2050. It is characterized by abnormal extracellular accumulation of amyloid‑beta (Aβ) peptide and intracellular neurofibrillary tangles (NFTs) that result in neuro‑inflammation, synaptic dysfunction, neurotransmitter imbalance, neuronal loss, and dendritic changes. A hypothesis of neurotrophic factor (NTF) involvement in neurodegenerative diseases and their potential as a therapeutic tool has emerged. There are wide information gaps on this topic. ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Joachim Hr L übke Faezeh Idoon Mina Mohasel-Roodi Fatemeh Alipour Javad Hami Alireza Ehteshampour Hamideh Mostafaee Akram Sadeghi Source Type: research

Morinda citrifolia Linn. fruit extract mitigates heroin seeking behavior in mice
This study demonstrates that MMC attenuated heroin seeking at different phases of drug self‑administration in a mouse modified runway model.PMID:35014982 (Source: Acta Neurobiologiae Experimentalis)
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Vijayapandi Pandy Yasmin Khan Durga Priyanka Yarlagadda Sri Pravallika Tatinada Source Type: research

Combinations of polyphenols disaggregate A β1‑42 by passing through in vitro blood brain barrier developed by endothelium, astrocyte, and differentiated SH‑SY5Y cells
Acta Neurobiol Exp (Wars). 2021;81(4):335-349.ABSTRACTDisaggregation of amyloid βeta (Aβ) is considered as one of the promising therapeutic strategies for Alzheimer's disease. Polyphenols are promising molecules for the disaggregation of Aβ. However, in order to find a potential therapeutic candidate, the in vitro analyses need to be performed on a model that mimics the blood‑brain barrier (BBB) as much as possible. Therefore, we aimed to establish an in vitro BBB representative transwell system by using differentiated human neuroblastoma (SH‑SY5Y), cerebral microvascular endothelial, and astrocyte cells...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Belgin Sert Serdar Tu ğba Erkmen Semra Ko çtürk Source Type: research

Synergistic analgesic effect of morphine and tramadol in non ‑sensitized and morphine‑sensitized mice: an isobolographic study
Acta Neurobiol Exp (Wars). 2021;81(4):350-361.ABSTRACTMorphine and tramadol are the opioid analgesic drugs acting via activation of μ‑opioid receptors. It is important to understand which mechanism (synergistic or additive anti‑nociceptive activity) induced potent anti‑nociceptive effect by co‑administration of morphine and tramadol. Identification of new strategies that can potentiate analgesic effects of opioids will be good therapeutic approaches for pain relief. To this aim, male mice were cannulated in the left ventricle by a stereotaxic instrument. A tail‑flick test was used to record the pain threshold....
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Fatemeh Khakpai Fatemeh Nedaei Faezeh Shahini Mohammad-Reza Zarrindast Source Type: research

Knockdown of NEAT1 induced microglial M2 polarization via miR ‑374a‑5p/NFAT5 axis to inhibit inflammatory response caused by OGD/R
Acta Neurobiol Exp (Wars). 2021;81(4):362-374.ABSTRACTThe long non‑coding RNAs (lncRNAs) have been important regulators for the progression of ischemic‑induced stroke. We aim to study the role of the lncRNA nuclear enriched abundant transcript 1 (NEAT1) in oxygen and glucose deprivation/reoxygenation (OGD/R) treated microglia. OGD/R injury of CHME5 cells was used as an in vitro stroke model. qRT‑PCR analysis was performed to examine NEAT1, miR‑374a‑5p, nuclear factor of activated T cells 5 (NFAT5) and cytokines. Western blot assay detected protein levels of NFAT5 and microglia markers. The concentration of cytoki...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Xiao-Wen Lian Bin Luo Source Type: research

Lipoic acid alleviates LPS ‑evoked PC12 cell damage by targeting p53 and inactivating the NF‑κB pathway
Acta Neurobiol Exp (Wars). 2021;81(4):375-385.ABSTRACTLipoic acid (LA) exerts several beneficial effects including anti‑inflammatory and antioxidant activity. This research aims to explore the function and mechanisms of LA on lipopolysaccharide (LPS)‑induced PC12 cells. PC12 cells stimulated by LPS were used to mimic an in vitro inflammatory model of Parkinson's disease. Cell toxicity was determined by a cell counting kit‑8 assay after various treatments. The concentrations of tumor necrosis factor (TNF-α), interleukin (IL)‑1β and IL‑6 were analyzed by an ELISA kit. The effects of LA on cell apoptosis ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Jieping Mao Hua Gao Wen Bai Haibo Zeng Yafang Ren Yu Liu Xinling Yang Source Type: research

Greater occipital nerve block efficacy in COVID ‑19 associated headache: a preliminary study
Acta Neurobiol Exp (Wars). 2021;81(4):386-392.ABSTRACTCOVID‑19 is an infection caused by the new coronavirus SARS‑CoV‑2. Headache is one of the most common neurological findings. In the treatment of various chronic headaches, the greater occipital nerve (GON) block is often used as a safe and effective method. The aim of our study was to investigate the effectiveness of the GON block in the treatment of headaches observed in COVID‑19 patients. Between March and May 2020, 27 patients (with laboratory‑confirmed 2019‑nCoV infection by next‑generation sequencing confirmation of real‑time PCR) that had moderate ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Ömer Karadaş Hakan Levent G ül Bilgin Öztürk Ali Riza Sonkaya Ak çay Övünç Özön Javid Shafiyev Ender Sir Source Type: research

Identification of traditional Japanese Kampo medicines and crude drugs that upregulate brain ‑derived neurotrophic factor in human peripheral cells
Acta Neurobiol Exp (Wars). 2021;81(4):393-404.ABSTRACTThe neurotrophic hypothesis of depression, which suggests that decreased hippocampal brain‑derived neurotrophic factor (BDNF) levels cause depression, has become increasingly popular. BDNF, a member of the neurotrophin family, promotes neuronal differentiation and survival. BDNF is synthesized in various peripheral tissues, as well as in the brain. Considering that peripheral BDNF can be transported into the brain across the blood‑brain barrier, substances with the ability to upregulate BDNF activity in peripheral tissues may be useful in the management of depressio...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Kensuke Nakajima Shinya Okubo Shigeru Oiso Source Type: research

Annexin A3: a newly identified player in store ‑operated calcium entry
Acta Neurobiol Exp (Wars). 2021;81(4):307-313.ABSTRACTStore‑operated calcium entry (SOCE) is important for refilling endoplasmic reticulum (ER) Ca2+ stores and Ca2+ signaling. Extracellular Ca2+ is conducted by highly Ca2+‑selective Orai channels that are activated by stromal interaction molecule proteins (STIMs), which are sensitive ER Ca2+ sensors. We found an approximately five‑fold increase in annexin A3a (anxa3a) expression levels in stim2b knockout zebrafish. The present study investigated whether annexin A3 protein is involved in SOCE. We used Ca2+ imaging and electrophysiological recordings to determine the e...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Agnieszka Dyrda Jacek Kuznicki Lukasz Majewski Source Type: research

Neurotrophic factors in Alzheimer's disease: pathogenesis and therapy
Acta Neurobiol Exp (Wars). 2021;81(4):314-327.ABSTRACTAlzheimer's disease (AD) is a common neurodegenerative disease with a prevalence estimated to reach 115 million by 2050. It is characterized by abnormal extracellular accumulation of amyloid‑beta (Aβ) peptide and intracellular neurofibrillary tangles (NFTs) that result in neuro‑inflammation, synaptic dysfunction, neurotransmitter imbalance, neuronal loss, and dendritic changes. A hypothesis of neurotrophic factor (NTF) involvement in neurodegenerative diseases and their potential as a therapeutic tool has emerged. There are wide information gaps on this topic. ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Joachim Hr L übke Faezeh Idoon Mina Mohasel-Roodi Fatemeh Alipour Javad Hami Alireza Ehteshampour Hamideh Mostafaee Akram Sadeghi Source Type: research

Morinda citrifolia Linn. fruit extract mitigates heroin seeking behavior in mice
This study demonstrates that MMC attenuated heroin seeking at different phases of drug self‑administration in a mouse modified runway model.PMID:35014982 (Source: Acta Neurobiologiae Experimentalis)
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Vijayapandi Pandy Yasmin Khan Durga Priyanka Yarlagadda Sri Pravallika Tatinada Source Type: research

Combinations of polyphenols disaggregate A β1‑42 by passing through in vitro blood brain barrier developed by endothelium, astrocyte, and differentiated SH‑SY5Y cells
Acta Neurobiol Exp (Wars). 2021;81(4):335-349.ABSTRACTDisaggregation of amyloid βeta (Aβ) is considered as one of the promising therapeutic strategies for Alzheimer's disease. Polyphenols are promising molecules for the disaggregation of Aβ. However, in order to find a potential therapeutic candidate, the in vitro analyses need to be performed on a model that mimics the blood‑brain barrier (BBB) as much as possible. Therefore, we aimed to establish an in vitro BBB representative transwell system by using differentiated human neuroblastoma (SH‑SY5Y), cerebral microvascular endothelial, and astrocyte cells...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Belgin Sert Serdar Tu ğba Erkmen Semra Ko çtürk Source Type: research

Synergistic analgesic effect of morphine and tramadol in non ‑sensitized and morphine‑sensitized mice: an isobolographic study
Acta Neurobiol Exp (Wars). 2021;81(4):350-361.ABSTRACTMorphine and tramadol are the opioid analgesic drugs acting via activation of μ‑opioid receptors. It is important to understand which mechanism (synergistic or additive anti‑nociceptive activity) induced potent anti‑nociceptive effect by co‑administration of morphine and tramadol. Identification of new strategies that can potentiate analgesic effects of opioids will be good therapeutic approaches for pain relief. To this aim, male mice were cannulated in the left ventricle by a stereotaxic instrument. A tail‑flick test was used to record the pain threshold....
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Fatemeh Khakpai Fatemeh Nedaei Faezeh Shahini Mohammad-Reza Zarrindast Source Type: research

Knockdown of NEAT1 induced microglial M2 polarization via miR ‑374a‑5p/NFAT5 axis to inhibit inflammatory response caused by OGD/R
Acta Neurobiol Exp (Wars). 2021;81(4):362-374.ABSTRACTThe long non‑coding RNAs (lncRNAs) have been important regulators for the progression of ischemic‑induced stroke. We aim to study the role of the lncRNA nuclear enriched abundant transcript 1 (NEAT1) in oxygen and glucose deprivation/reoxygenation (OGD/R) treated microglia. OGD/R injury of CHME5 cells was used as an in vitro stroke model. qRT‑PCR analysis was performed to examine NEAT1, miR‑374a‑5p, nuclear factor of activated T cells 5 (NFAT5) and cytokines. Western blot assay detected protein levels of NFAT5 and microglia markers. The concentration of cytoki...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Xiao-Wen Lian Bin Luo Source Type: research

Lipoic acid alleviates LPS ‑evoked PC12 cell damage by targeting p53 and inactivating the NF‑κB pathway
Acta Neurobiol Exp (Wars). 2021;81(4):375-385.ABSTRACTLipoic acid (LA) exerts several beneficial effects including anti‑inflammatory and antioxidant activity. This research aims to explore the function and mechanisms of LA on lipopolysaccharide (LPS)‑induced PC12 cells. PC12 cells stimulated by LPS were used to mimic an in vitro inflammatory model of Parkinson's disease. Cell toxicity was determined by a cell counting kit‑8 assay after various treatments. The concentrations of tumor necrosis factor (TNF-α), interleukin (IL)‑1β and IL‑6 were analyzed by an ELISA kit. The effects of LA on cell apoptosis ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Jieping Mao Hua Gao Wen Bai Haibo Zeng Yafang Ren Yu Liu Xinling Yang Source Type: research

Greater occipital nerve block efficacy in COVID ‑19 associated headache: a preliminary study
Acta Neurobiol Exp (Wars). 2021;81(4):386-392.ABSTRACTCOVID‑19 is an infection caused by the new coronavirus SARS‑CoV‑2. Headache is one of the most common neurological findings. In the treatment of various chronic headaches, the greater occipital nerve (GON) block is often used as a safe and effective method. The aim of our study was to investigate the effectiveness of the GON block in the treatment of headaches observed in COVID‑19 patients. Between March and May 2020, 27 patients (with laboratory‑confirmed 2019‑nCoV infection by next‑generation sequencing confirmation of real‑time PCR) that had moderate ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Ömer Karadaş Hakan Levent G ül Bilgin Öztürk Ali Riza Sonkaya Ak çay Övünç Özön Javid Shafiyev Ender Sir Source Type: research

Identification of traditional Japanese Kampo medicines and crude drugs that upregulate brain ‑derived neurotrophic factor in human peripheral cells
Acta Neurobiol Exp (Wars). 2021;81(4):393-404.ABSTRACTThe neurotrophic hypothesis of depression, which suggests that decreased hippocampal brain‑derived neurotrophic factor (BDNF) levels cause depression, has become increasingly popular. BDNF, a member of the neurotrophin family, promotes neuronal differentiation and survival. BDNF is synthesized in various peripheral tissues, as well as in the brain. Considering that peripheral BDNF can be transported into the brain across the blood‑brain barrier, substances with the ability to upregulate BDNF activity in peripheral tissues may be useful in the management of depressio...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Kensuke Nakajima Shinya Okubo Shigeru Oiso Source Type: research

Annexin A3: a newly identified player in store ‑operated calcium entry
Acta Neurobiol Exp (Wars). 2021;81(4):307-313.ABSTRACTStore‑operated calcium entry (SOCE) is important for refilling endoplasmic reticulum (ER) Ca2+ stores and Ca2+ signaling. Extracellular Ca2+ is conducted by highly Ca2+‑selective Orai channels that are activated by stromal interaction molecule proteins (STIMs), which are sensitive ER Ca2+ sensors. We found an approximately five‑fold increase in annexin A3a (anxa3a) expression levels in stim2b knockout zebrafish. The present study investigated whether annexin A3 protein is involved in SOCE. We used Ca2+ imaging and electrophysiological recordings to determine the e...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Agnieszka Dyrda Jacek Kuznicki Lukasz Majewski Source Type: research

Neurotrophic factors in Alzheimer's disease: pathogenesis and therapy
Acta Neurobiol Exp (Wars). 2021;81(4):314-327.ABSTRACTAlzheimer's disease (AD) is a common neurodegenerative disease with a prevalence estimated to reach 115 million by 2050. It is characterized by abnormal extracellular accumulation of amyloid‑beta (Aβ) peptide and intracellular neurofibrillary tangles (NFTs) that result in neuro‑inflammation, synaptic dysfunction, neurotransmitter imbalance, neuronal loss, and dendritic changes. A hypothesis of neurotrophic factor (NTF) involvement in neurodegenerative diseases and their potential as a therapeutic tool has emerged. There are wide information gaps on this topic. ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Joachim Hr L übke Faezeh Idoon Mina Mohasel-Roodi Fatemeh Alipour Javad Hami Alireza Ehteshampour Hamideh Mostafaee Akram Sadeghi Source Type: research

Morinda citrifolia Linn. fruit extract mitigates heroin seeking behavior in mice
This study demonstrates that MMC attenuated heroin seeking at different phases of drug self‑administration in a mouse modified runway model.PMID:35014982 (Source: Acta Neurobiologiae Experimentalis)
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Vijayapandi Pandy Yasmin Khan Durga Priyanka Yarlagadda Sri Pravallika Tatinada Source Type: research

Combinations of polyphenols disaggregate A β1‑42 by passing through in vitro blood brain barrier developed by endothelium, astrocyte, and differentiated SH‑SY5Y cells
Acta Neurobiol Exp (Wars). 2021;81(4):335-349.ABSTRACTDisaggregation of amyloid βeta (Aβ) is considered as one of the promising therapeutic strategies for Alzheimer's disease. Polyphenols are promising molecules for the disaggregation of Aβ. However, in order to find a potential therapeutic candidate, the in vitro analyses need to be performed on a model that mimics the blood‑brain barrier (BBB) as much as possible. Therefore, we aimed to establish an in vitro BBB representative transwell system by using differentiated human neuroblastoma (SH‑SY5Y), cerebral microvascular endothelial, and astrocyte cells...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Belgin Sert Serdar Tu ğba Erkmen Semra Ko çtürk Source Type: research

Synergistic analgesic effect of morphine and tramadol in non ‑sensitized and morphine‑sensitized mice: an isobolographic study
Acta Neurobiol Exp (Wars). 2021;81(4):350-361.ABSTRACTMorphine and tramadol are the opioid analgesic drugs acting via activation of μ‑opioid receptors. It is important to understand which mechanism (synergistic or additive anti‑nociceptive activity) induced potent anti‑nociceptive effect by co‑administration of morphine and tramadol. Identification of new strategies that can potentiate analgesic effects of opioids will be good therapeutic approaches for pain relief. To this aim, male mice were cannulated in the left ventricle by a stereotaxic instrument. A tail‑flick test was used to record the pain threshold....
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Fatemeh Khakpai Fatemeh Nedaei Faezeh Shahini Mohammad-Reza Zarrindast Source Type: research

Knockdown of NEAT1 induced microglial M2 polarization via miR ‑374a‑5p/NFAT5 axis to inhibit inflammatory response caused by OGD/R
Acta Neurobiol Exp (Wars). 2021;81(4):362-374.ABSTRACTThe long non‑coding RNAs (lncRNAs) have been important regulators for the progression of ischemic‑induced stroke. We aim to study the role of the lncRNA nuclear enriched abundant transcript 1 (NEAT1) in oxygen and glucose deprivation/reoxygenation (OGD/R) treated microglia. OGD/R injury of CHME5 cells was used as an in vitro stroke model. qRT‑PCR analysis was performed to examine NEAT1, miR‑374a‑5p, nuclear factor of activated T cells 5 (NFAT5) and cytokines. Western blot assay detected protein levels of NFAT5 and microglia markers. The concentration of cytoki...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Xiao-Wen Lian Bin Luo Source Type: research

Lipoic acid alleviates LPS ‑evoked PC12 cell damage by targeting p53 and inactivating the NF‑κB pathway
Acta Neurobiol Exp (Wars). 2021;81(4):375-385.ABSTRACTLipoic acid (LA) exerts several beneficial effects including anti‑inflammatory and antioxidant activity. This research aims to explore the function and mechanisms of LA on lipopolysaccharide (LPS)‑induced PC12 cells. PC12 cells stimulated by LPS were used to mimic an in vitro inflammatory model of Parkinson's disease. Cell toxicity was determined by a cell counting kit‑8 assay after various treatments. The concentrations of tumor necrosis factor (TNF-α), interleukin (IL)‑1β and IL‑6 were analyzed by an ELISA kit. The effects of LA on cell apoptosis ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Jieping Mao Hua Gao Wen Bai Haibo Zeng Yafang Ren Yu Liu Xinling Yang Source Type: research

Greater occipital nerve block efficacy in COVID ‑19 associated headache: a preliminary study
Acta Neurobiol Exp (Wars). 2021;81(4):386-392.ABSTRACTCOVID‑19 is an infection caused by the new coronavirus SARS‑CoV‑2. Headache is one of the most common neurological findings. In the treatment of various chronic headaches, the greater occipital nerve (GON) block is often used as a safe and effective method. The aim of our study was to investigate the effectiveness of the GON block in the treatment of headaches observed in COVID‑19 patients. Between March and May 2020, 27 patients (with laboratory‑confirmed 2019‑nCoV infection by next‑generation sequencing confirmation of real‑time PCR) that had moderate ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Ömer Karadaş Hakan Levent G ül Bilgin Öztürk Ali Riza Sonkaya Ak çay Övünç Özön Javid Shafiyev Ender Sir Source Type: research

Identification of traditional Japanese Kampo medicines and crude drugs that upregulate brain ‑derived neurotrophic factor in human peripheral cells
Acta Neurobiol Exp (Wars). 2021;81(4):393-404.ABSTRACTThe neurotrophic hypothesis of depression, which suggests that decreased hippocampal brain‑derived neurotrophic factor (BDNF) levels cause depression, has become increasingly popular. BDNF, a member of the neurotrophin family, promotes neuronal differentiation and survival. BDNF is synthesized in various peripheral tissues, as well as in the brain. Considering that peripheral BDNF can be transported into the brain across the blood‑brain barrier, substances with the ability to upregulate BDNF activity in peripheral tissues may be useful in the management of depressio...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Kensuke Nakajima Shinya Okubo Shigeru Oiso Source Type: research

Annexin A3: a newly identified player in store ‑operated calcium entry
Acta Neurobiol Exp (Wars). 2021;81(4):307-313.ABSTRACTStore‑operated calcium entry (SOCE) is important for refilling endoplasmic reticulum (ER) Ca2+ stores and Ca2+ signaling. Extracellular Ca2+ is conducted by highly Ca2+‑selective Orai channels that are activated by stromal interaction molecule proteins (STIMs), which are sensitive ER Ca2+ sensors. We found an approximately five‑fold increase in annexin A3a (anxa3a) expression levels in stim2b knockout zebrafish. The present study investigated whether annexin A3 protein is involved in SOCE. We used Ca2+ imaging and electrophysiological recordings to determine the e...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Agnieszka Dyrda Jacek Kuznicki Lukasz Majewski Source Type: research

Neurotrophic factors in Alzheimer's disease: pathogenesis and therapy
Acta Neurobiol Exp (Wars). 2021;81(4):314-327.ABSTRACTAlzheimer's disease (AD) is a common neurodegenerative disease with a prevalence estimated to reach 115 million by 2050. It is characterized by abnormal extracellular accumulation of amyloid‑beta (Aβ) peptide and intracellular neurofibrillary tangles (NFTs) that result in neuro‑inflammation, synaptic dysfunction, neurotransmitter imbalance, neuronal loss, and dendritic changes. A hypothesis of neurotrophic factor (NTF) involvement in neurodegenerative diseases and their potential as a therapeutic tool has emerged. There are wide information gaps on this topic. ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Joachim Hr L übke Faezeh Idoon Mina Mohasel-Roodi Fatemeh Alipour Javad Hami Alireza Ehteshampour Hamideh Mostafaee Akram Sadeghi Source Type: research

Morinda citrifolia Linn. fruit extract mitigates heroin seeking behavior in mice
This study demonstrates that MMC attenuated heroin seeking at different phases of drug self‑administration in a mouse modified runway model.PMID:35014982 (Source: Acta Neurobiologiae Experimentalis)
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Vijayapandi Pandy Yasmin Khan Durga Priyanka Yarlagadda Sri Pravallika Tatinada Source Type: research

Combinations of polyphenols disaggregate A β1‑42 by passing through in vitro blood brain barrier developed by endothelium, astrocyte, and differentiated SH‑SY5Y cells
Acta Neurobiol Exp (Wars). 2021;81(4):335-349.ABSTRACTDisaggregation of amyloid βeta (Aβ) is considered as one of the promising therapeutic strategies for Alzheimer's disease. Polyphenols are promising molecules for the disaggregation of Aβ. However, in order to find a potential therapeutic candidate, the in vitro analyses need to be performed on a model that mimics the blood‑brain barrier (BBB) as much as possible. Therefore, we aimed to establish an in vitro BBB representative transwell system by using differentiated human neuroblastoma (SH‑SY5Y), cerebral microvascular endothelial, and astrocyte cells...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Belgin Sert Serdar Tu ğba Erkmen Semra Ko çtürk Source Type: research

Synergistic analgesic effect of morphine and tramadol in non ‑sensitized and morphine‑sensitized mice: an isobolographic study
Acta Neurobiol Exp (Wars). 2021;81(4):350-361.ABSTRACTMorphine and tramadol are the opioid analgesic drugs acting via activation of μ‑opioid receptors. It is important to understand which mechanism (synergistic or additive anti‑nociceptive activity) induced potent anti‑nociceptive effect by co‑administration of morphine and tramadol. Identification of new strategies that can potentiate analgesic effects of opioids will be good therapeutic approaches for pain relief. To this aim, male mice were cannulated in the left ventricle by a stereotaxic instrument. A tail‑flick test was used to record the pain threshold....
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Fatemeh Khakpai Fatemeh Nedaei Faezeh Shahini Mohammad-Reza Zarrindast Source Type: research

Knockdown of NEAT1 induced microglial M2 polarization via miR ‑374a‑5p/NFAT5 axis to inhibit inflammatory response caused by OGD/R
Acta Neurobiol Exp (Wars). 2021;81(4):362-374.ABSTRACTThe long non‑coding RNAs (lncRNAs) have been important regulators for the progression of ischemic‑induced stroke. We aim to study the role of the lncRNA nuclear enriched abundant transcript 1 (NEAT1) in oxygen and glucose deprivation/reoxygenation (OGD/R) treated microglia. OGD/R injury of CHME5 cells was used as an in vitro stroke model. qRT‑PCR analysis was performed to examine NEAT1, miR‑374a‑5p, nuclear factor of activated T cells 5 (NFAT5) and cytokines. Western blot assay detected protein levels of NFAT5 and microglia markers. The concentration of cytoki...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Xiao-Wen Lian Bin Luo Source Type: research

Lipoic acid alleviates LPS ‑evoked PC12 cell damage by targeting p53 and inactivating the NF‑κB pathway
Acta Neurobiol Exp (Wars). 2021;81(4):375-385.ABSTRACTLipoic acid (LA) exerts several beneficial effects including anti‑inflammatory and antioxidant activity. This research aims to explore the function and mechanisms of LA on lipopolysaccharide (LPS)‑induced PC12 cells. PC12 cells stimulated by LPS were used to mimic an in vitro inflammatory model of Parkinson's disease. Cell toxicity was determined by a cell counting kit‑8 assay after various treatments. The concentrations of tumor necrosis factor (TNF-α), interleukin (IL)‑1β and IL‑6 were analyzed by an ELISA kit. The effects of LA on cell apoptosis ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Jieping Mao Hua Gao Wen Bai Haibo Zeng Yafang Ren Yu Liu Xinling Yang Source Type: research

Greater occipital nerve block efficacy in COVID ‑19 associated headache: a preliminary study
Acta Neurobiol Exp (Wars). 2021;81(4):386-392.ABSTRACTCOVID‑19 is an infection caused by the new coronavirus SARS‑CoV‑2. Headache is one of the most common neurological findings. In the treatment of various chronic headaches, the greater occipital nerve (GON) block is often used as a safe and effective method. The aim of our study was to investigate the effectiveness of the GON block in the treatment of headaches observed in COVID‑19 patients. Between March and May 2020, 27 patients (with laboratory‑confirmed 2019‑nCoV infection by next‑generation sequencing confirmation of real‑time PCR) that had moderate ...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Ömer Karadaş Hakan Levent G ül Bilgin Öztürk Ali Riza Sonkaya Ak çay Övünç Özön Javid Shafiyev Ender Sir Source Type: research

Identification of traditional Japanese Kampo medicines and crude drugs that upregulate brain ‑derived neurotrophic factor in human peripheral cells
Acta Neurobiol Exp (Wars). 2021;81(4):393-404.ABSTRACTThe neurotrophic hypothesis of depression, which suggests that decreased hippocampal brain‑derived neurotrophic factor (BDNF) levels cause depression, has become increasingly popular. BDNF, a member of the neurotrophin family, promotes neuronal differentiation and survival. BDNF is synthesized in various peripheral tissues, as well as in the brain. Considering that peripheral BDNF can be transported into the brain across the blood‑brain barrier, substances with the ability to upregulate BDNF activity in peripheral tissues may be useful in the management of depressio...
Source: Acta Neurobiologiae Experimentalis - January 11, 2022 Category: Neurology Authors: Kensuke Nakajima Shinya Okubo Shigeru Oiso Source Type: research

An A β3‑10‑KLH vaccine decreases Aβ plaques and astrocytes and microglia activation in the brain of APP/PS1 transgenic mice
In conclusion, early immunotherapy with an Aβ vaccine reduces the activation of glial cells and deposition of Aβ plaque in the brain of transgenic mice.PMID:34672292 | DOI:10.21307/ane‑2021‑020 (Source: Acta Neurobiologiae Experimentalis)
Source: Acta Neurobiologiae Experimentalis - October 21, 2021 Category: Neurology Authors: Yang Wang Bing Xu Jin Zhou Jinchun Wang Guoqing Wang Yunpeng Cao Source Type: research

Brain ‑derived neurotrophic factor and nitric oxide contribute to protective effects of rosiglitazone on learning and memory in hypothyroid rats
Acta Neurobiol Exp (Wars). 2021;81(3):218-232. doi: 10.21307/ane-2021-021.ABSTRACTThe effects of the well‑known peroxisome proliferator‑activated receptor gamma (PPAR-γ) agonist rosiglitazone (Rosi) on brain‑derived neurotrophic factor (BDNF), nitric oxide (NO), and learning and memory were investigated in hypothyroid rats. Hypothyroidism was induced in immature Wistar rats by administration of propylthiouracil in drinking water. Rats were divided into four groups: control, hypothyroid, and hypothyroid treated with Rosi at doses of 2 mg/kg or 4 mg/kg. Memory was then assessed by the Morris water maze (MWM) and ...
Source: Acta Neurobiologiae Experimentalis - October 21, 2021 Category: Neurology Authors: Yousef Baghcheghi Farimah Beheshti Hossein Salmani Mahmoud Hosseini Source Type: research

Inhibition of ionotropic GluR signaling preserves oligodendrocyte lineage and myelination in an ex vivo rat model of white matter ischemic injury
Acta Neurobiol Exp (Wars). 2021;81(3):233-248. doi: 10.21307/ane-2021-022.ABSTRACTPreterm infants have a high risk of neonatal white matter injury (WMI). WMI leads to reduced myelination, inflammation, and clinical neurodevelopmental deficits for which there are no effective treatments. Ionotropic glutamate receptor (iGluR) induced excitotoxicity contributes to oligodendrocyte (OL) lineage cell loss and demyelination in brain models of neonatal and adult WMI. Here, we hypothesized that simulated ischemia (oxygen‑glucose deprivation) damages white matter via activation of iGluR signaling, and that iGluR inhibition shortly...
Source: Acta Neurobiologiae Experimentalis - October 21, 2021 Category: Neurology Authors: Mohamed A Al-Griw Michael G Salter Ian C Wood Source Type: research

The immune system and autism spectrum disorder: association and therapeutic challenges
This study provides a review of the autoimmune involvement in the pathogenesis of ASD. The\r\nmicrobiome, the representative of the innate immune system in the central nervous system (CNS), plays a critical role in triggering inflammation. Besides, a bidirectional communicational pathway between the CNS and the intestine called the gut‑brain‑axis is linked to the development of ASD. Moreover, the higher plasma level of pro‑inflammatory cytokines in ASD patients and the higher prevalence of autoimmune disorders in the first‑degree family members of affected persons are other clues of the immune system involvement in...
Source: Acta Neurobiologiae Experimentalis - October 21, 2021 Category: Neurology Authors: Arash Heidari Yasna Rostam-Abadi Nima Rezaei Source Type: research

Effects of scopolamine treatment and consequent convulsion development in c ‑fos expression in fed, fasted, and refed mice
Acta Neurobiol Exp (Wars). 2021;81(3):264-270. doi: 10.21307/ane-2021-024.ABSTRACTFasting, anticholinergics, and seizures affect c‑fos activation in the brain. Additionally, antimuscarinic treated fasted animals develop convulsion soon after re‑feeding. Therefore, we assessed whether c‑fos expression changes in fed, fasting, and refed animals and how scopolamine treatment affects these changes. We further assessed whether there is a change in c‑fos expression after convulsions. For this purpose, BALB/c mice fasted for 1, 3, 6, 12, 24 and 48 h periods were used. The animals were treated with saline or scopolamine. H...
Source: Acta Neurobiologiae Experimentalis - October 21, 2021 Category: Neurology Authors: Asli Zengin Turkmen Asiye Nurten Mine Erguven Emine Bilge Source Type: research