Esm-1 mediates transcriptional polarization associated with diabetic kidney disease

Am J Physiol Renal Physiol. 2024 Apr 11. doi: 10.1152/ajprenal.00419.2023. Online ahead of print.ABSTRACTEsm-1, endothelial cell-specific molecule-1, is a susceptibility gene for diabetic kidney disease (DKD) and is a secreted proteoglycan, with notable expression in kidney, which attenuates inflammation and albuminuria. However, little is known about Esm1 expression in mature tissues in the presence or absence of diabetes. We utilized publicly available single-cell RNA sequencing data to characterize Esm1 expression in 27,786 renal endothelial cells (RECs) obtained from four human and three mouse databases. We validated our findings using bulk transcriptome data from 20 healthy subjects and 41 patients with DKD and using RNAscope. In both mice and humans, Esm1 is expressed in a subset of all RECs types and represents a minority of glomerular RECs. In patients, Esm1(+) cells exhibit conserved enrichment for blood vessel development genes. With diabetes, these cells are fewer in number and shift expression towards chemotaxis pathways. Esm1 correlates with a majority of genes within these pathways, delineating a glomerular transcriptional polarization reflected by the magnitude of Esm1 deficiency. Diabetes correlates with lower Esm1 expression and with changes in the functional characterization of Esm1(+) cells. Esm1 thus appears as a marker for glomerular transcriptional polarization in DKD.PMID:38601985 | DOI:10.1152/ajprenal.00419.2023
Source: American Journal of Physiology. Renal Physiology - Category: Physiology Authors: Source Type: research